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The articles database is a growing compilation of wide-ranging articles from bioanalysis – including primary research, reviews, White Papers, event reports, interviews and more – drawn from a range of sources.
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Strategies to address drug interaction potential for antibody–drug conjugates in clinical developmentAntibody–drug conjugates (ADCs) are a unique class of therapeutic proteins with both small and large molecular components. In vivo, ADCs are processed to multiple clinically relevant analytes,...Posted 23/05/2013 (-1 days ago)
Bioanalysis of antibody–drug conjugates: American Association of Pharmaceutical Scientists Antibody–Drug Conjugate Working Group position paperAntibody–drug conjugates (ADCs) typically consist of a cytotoxic drug covalently bound to an antibody by a linker. hese conjugates have the potential to substantially improve efficacy and...Posted 23/05/2013 (-1 days ago)
Bioanalysis special focus issue on antibody–drug conjugates“As knowledge emerges from the breadth of antibody–drug conjugates currently in development within the industry, it will help to shape best practices for bioanalysis of...Posted 22/05/2013 (today)
Interview with Leroy Hood (Institute of Systems Biology)Leroy Hood received an MD from Johns Hopkins and a PhD from Caltech. He was a professor at Caltech for 22 years, at the University of Washington for 8 years and has been President and co-founder of...Posted 16/05/2013 (6 days ago)- Interview with Steve Lowes (Quintiles)Steve Lowes is Vice President of Scientific for Quintiles’ Bioanalytical & ADME Labs. He has focused on quantitative bioanalysis since his postgraduate work at the Open University...Posted 09/05/2013 (2 weeks ago)
- Foreword to J Burrows articles“Altering the status quo always takes someone putting something out into the community and requires others who are willing to give it a try. This is my hope and my challenge to our...Posted 09/05/2013 (2 weeks ago)
Determination of urinary 6-mercaptopurine and three of its metabolites by HPLC–UV coupled with the iodine–azide reactionBackground: The presented method is able to determine 6-mercaptopurine (6-MP), 6-thioguanine, 6-mercaptopurine riboside and 6-thioguanine riboside in urine, and is thereby dedicated to control of...Posted 09/05/2013 (2 weeks ago)- Hair as an alternative matrix in bioanalysisAlternative matrices are steadily gaining recognition as biological samples for toxicological analyses. Hair presents many advantages over traditional matrices, such as urine and blood, since it...Posted 09/05/2013 (2 weeks ago)
- How the accelerator MS community are trying to fit into the regulatory framework: are the BMV guidances relevant?“…it is to be hoped that as a result of the efforts of the European Bioanalysis Forum and initiatives such as the Global Bioanalysis Consortium, future revisions of the Bioanalytical...Posted 02/05/2013 (3 weeks ago)
- Molecularly imprinted SPE combined with dispersive liquid–liquid microextraction for selective analysis of telmisartan in biological and formulation samplesBackground: The present communication describes the combination of molecularly imprinted SPE and dispersive liquid–liquid microextraction for the selective preconcentration and determination of...Posted 18/04/2013 (1 month ago)
- Quantification of the sixth DNA base 5-hydroxymethylcytosine in colorectal cancer tissue and C-26 cell lineBackground: DNA methylation at the five position of cytosine is well recognized as an important epigenetic modification in human health and disease. Recent evidences demonstrated that...Posted 18/04/2013 (1 month ago)
- Is the tiered immunogenicity testing of biologics the adequate approach in preclinical development?“…immunogenicity testing remains a case-by-case study, in which preclinical assessment can deliver pivotal dataabout the immunogenic potential of a biologic.”
Posted 16/04/2013 (1 month ago)
Webinar: Addressing the Needs of Bio-analysis with New SPE Technologies – SOLAPosted 05/04/2013 (2 months ago)
- Investigating Matrix Interference in Analysis of Antiarrhythmic Cardiac Drugs in PlasmaPosted 28/03/2013 (2 months ago)
- Interview with Ron Bowsher (AIT BioSciences, B2S Consulting, & B2S Labs)In March 2013, Ronald Bowsher (AIT BioSciences) talks to Alexandra Sklan at Bioanalysis about his current bioanalytical career, his involvement in the American Association of Pharmaceutical...Posted 28/03/2013 (2 months ago)
- Hollow-fiber liquid-phase microextraction and chiral LC–MS/MS analysis of venlafaxine and its metabolites in plasmaBackground: An enantioselective analytical method was developed and validated for determination of venlafaxine and its metabolites O-desmethylvenlafaxine and N desmethylvenlafaxine in plasma samples....Posted 21/03/2013 (2 months ago)
Dispersive liquid–liquid microextraction of propranolol enantiomers from human plasma based on the solidification of a floating organic dropletBackground: A dispersive liquid–liquid microextraction based on the solidification of a floating organic droplet was developed and validated for the extraction of propranolol enantiomers from...Posted 21/03/2013 (2 months ago)- The European Bioanalysis Forum community’s evaluation, interpretation and implementation of the European Medicines Agency guideline on Bioanalytical Method ValidationThe European Medicines Agency’s (EMA) 2011 guideline on bioanalytical method validation (BMV) was evaluated and subsequently intensely discussed by the European Bioanalysis Forum (EBF) during a...Posted 19/03/2013 (2 months ago)
- Society Spotlight: China Bioanalysis ForumThe China Bioanalysis Forum (CBF), a volunteer and science focused group, was recently formed. The formation of CBF is a response to the urgent needs of updating current Chinese bioanalytical...Posted 19/03/2013 (2 months ago)
- Quantitative surface-assisted laser desorption/ionization–MS approaches for bioanalysis “…SALDI-MS using functionalized nanoparticles holds great potential of becoming a primary technique for protein analysis and for MS imaging. ”
Posted 19/03/2013 (2 months ago)
- Top ten: Critical topics in ensuring data quality in bioanalytical LC–MS method developmentPosted 15/03/2013 (2 months ago)
- Comparison of newly developed immuno-MS method with existing DELFIA® immunoassay for human chorionic gonadotropin determination in doping analysisBackground: The performance of a method for MS determination of human chorionic gonadotropin (hCG) was compared with a reference method currently used in World Anti-Doping Agency accredited doping...Posted 07/03/2013 (2 months ago)
- Analytical platform for glycomic characterization of recombinant erythropoietin biotherapeutics and biosimilars by MSBackground: Erythropoietin is a therapeutic glycoprotein that stimulates red blood cell production. The quality, safety and potency of recombinant erythropoietins are determined largely by their...Posted 07/03/2013 (2 months ago)
Conference Report: Bioanalysis-related topics presented at the International Conference and Exhibition on Biowaivers and BiosimilarsThis conference of approximately 30 professionals attracted a diverse group of attendees, whose expertise spanned the broad topics of development, production, testing and regulation of biosimilars....Posted 28/02/2013 (3 months ago)- European perspective on biosimilars “Although dogged by much controversy, the scientific approach adopted in the EU for approval of biosimilars to ensure patient safety has been sound.”
Posted 28/02/2013 (3 months ago)
- Analytical and bioanalytical assay challenges to support comparability studies for biosimilar drug development"..to ensure safety and efficacy of a biosimilar in comparison with its originator drug, preclinical and clinical comparabiltiy studies must be conducted according to the regulatory guidance for...Posted 28/02/2013 (3 months ago)
- Mini Focus: bioanalysis of biosimilarsPosted 28/02/2013 (3 months ago)
- Dispersive liquid–liquid microextraction based on solidification of floating organic droplet followed by spectrofluorimetry for determination of carvedilol in human plasmaBackground: Simple, chip and rapid analytical methods are required in biomedical analysis laboratories to support therapeutic drug monitoring units in hospitals. The present work aimed to provide...Posted 28/02/2013 (3 months ago)
- Ultrasensitive and automated 1 pg/ml fluticasone propionate assay in human plasma using LC–MS/MSBackground: To develop and validate an ultrasensitive bioanalytical assay for quantitation of fluticasone propionate in human plasma, aliquots of 0.6 ml of K3EDTA human plasma were treated with zinc...Posted 28/02/2013 (3 months ago)
- Strategic regulatory approaches for the qualification of a biomarker assay for safety useBiomarkers can be defined as key molecular or cellular events that link a specific biological event to a health outcome. As such, biomarkers play an important role in understanding the relationships...Posted 26/02/2013 (3 months ago)
- Host Cell Protein Workshop at the 2012 Annual Bioassay Meeting of the Biopharmaceutical Emerging Best Practices Association2012 Annual Bioassay Meeting of the Biopharmaceutical Emerging Best Practices Association
Lisbon, Portugal, 26–28 September 2012
Posted 26/02/2013 (3 months ago)
- A hitchhiker’s guide to outsourcing ADME studies: the inside of outsourcingAmerican Association of Pharmaceutical Scientists 2012 Annual Meeting and Exposition.
Chicago, IL, USA, 17 October 2012
Posted 26/02/2013 (3 months ago)
- 3D thinking: computational aids for the bioanalyst“The word ‘quantum’ immediately brings images of such scientific giants as Heisenberg and Schrodinger to mind. Yet we do not need to be a physicist to apply quantum mechanics on a...Posted 26/02/2013 (3 months ago)
- Interview with David Mitchell (AAPS)David Mitchell is president of Mitchell Pharmaceutical Consulting and has more than 18 years of pharmaceutical and biotechnology experience in clinical pharmacology, project leadership, and strategic...Posted 07/02/2013 (3 months ago)
- Antiretroviral bioanalysis methods of tissues and body biofluidsResearch in the many areas of HIV treatment, eradication and prevention has necessitated measurement of antiretroviral (ARV) concentrations in nontraditional specimen types. To determine the...Posted 19/02/2013 (3 months ago)
- Quantitative research of histone H3 acetylation levels of human hepatocellular carcinoma cellsBackground: Core histone H3 is a highly conserved protein in the cell nucleus, it goes through various post-translational modifications easily, and the state of the acetylation has clinical...Posted 19/02/2013 (3 months ago)
- Supported liquid extraction versus liquid–liquid extraction for sample preparation in LC–MS/MS-based bioanalysis“Due to its flow-through property and the large surface area of the interface between the aqueous and organic layer, SLE provides continuous and more efficient extraction than LLE does,...Posted 14/02/2013 (3 months ago)
- Conference Report: International harmonization of bioanalysis regulation: discussion in Global Bioanalysis Consortium harmonization teamsThe Japan Bioanalysis Forum was established in August 2011 by delegates from industry, regulators and academia. The forum was founded as the place for discussion on regulated bioanalysis in Japan,...Posted 14/02/2013 (3 months ago)
- Conference Report: Transforming drug discovery and development through innovation: past, present and futureThis annual meeting began in 1998 and was the first industry-led event to focus on the specific needs of industry researchers. The goal of Clinical and Pharmaceutical Solutions Through Analysis...Posted 14/02/2013 (3 months ago)
- Antibody-free PRISM–SRM for multiplexed protein quantification: is this the new competition for immunoassays in bioanalysis?“…PRISM–SRM affords a unique protein quantification technology for biomarker verification and systems biologystudies where a large number of proteins need to be quantified from a...Posted 14/02/2013 (3 months ago)
- Ex vivo conversion of prodrug prothionamide to its metabolite prothionamide sulfoxide with different extraction techniques and their estimation in human plasma by LC–MS/MSBackground: The present work reports an ex vivo conversion study of prothionamide to its active metabolite prothionamide sulfoxide in human plasma during sample preparation by three conventional...Posted 31/01/2013 (4 months ago)
- Enantioselective analysis of ranolazine and desmethyl ranolazine in microsomal medium using dispersive liquid–liquid microextraction and LC–MS/MSBackground: An enantioselective bioanalytical method using dispersive liquid–liquid microextraction (DLLME) and LC–MS/MS was developed for the chiral analysis of ranolazine (RNZ) and one...Posted 31/01/2013 (4 months ago)
Managing scientific, technical and regulatory innovation in regulated bioanalysis: a discussion paper from the European Bioanalysis ForumOn 12–13 June 2012, the European Bioanalysis Forum hosted its third Focus Meeting in Brussels (Belgium). At the meeting, a panel discussion was held on the hurdles that the bioanalytical...Posted 29/01/2013 (4 months ago)- Conference Report: Advances in clinical analysis 2012A report on the meeting organized by The Chromatographic Society and the Separation Science Group, Analytical Division of the Royal Society of Chemistry. Over 60 delegates and commercial exhibitors...Posted 29/01/2013 (4 months ago)
- Conference Report: Chemistry: a bridge to discoveryAmerican Chemical Society Regional Meetings are a convenient and economical route for students, faculty and industry representatives to come together and communicate scientific findings and ideas....Posted 29/01/2013 (4 months ago)
- The challenges of product- and process-related impurities to an evolving biopharmaceutical industry“The analytical technologies available for the monitoring of product- and process-related impurities in recombinant protein preparations have developed enormously in the last 10–20 years....Posted 29/01/2013 (4 months ago)
- In vivo electrochemical measurements: past, present and future“…one of the strengths of electrochemical measurements in living brain tissue is the ability to combine it with complementary methods. These methods include behavioral measurements,...Posted 29/01/2013 (4 months ago)
- Disposable pipette extraction for the simultaneous determination of biperiden and three antipsychotic drugs in human urine by GC–nitrogen phosphorus detectionBackground: Disposable pipette extraction with reversed-phase sorbent is proposed for the fast and simple GC determination of the antipsychotic drugs chlorpromazine, olanzapine, clozapine and...Posted 17/01/2013 (4 months ago)
- Iodine-129 microdosing for protein and peptide drug development: erythropoietin as a case studyBackground: Microdosing is a technique for studying the behavior of compounds in vivo at 1/100th of the dose of a test substance calculated, based on animal data, to yield a pharmacologic effect. In...Posted 17/01/2013 (4 months ago)
- LC–MS/MS in clinical laboratories “It should be understood that poorly developed, inadequately validated analytical methods, lack of QC and poorly qualified technical personnel will likely result in poorly harmonized...Posted 15/01/2013 (4 months ago)
- The future of microfluidic point-of-care diagnostic devices “To make this [personalized medicine] a reality requires the combination of already developed microfluidic components into point-of-care devices that are minimally invasive, accurate,...Posted 15/01/2013 (4 months ago)
- Applying biofluid metabonomic techniques to analyze the combined subchronic toxicity of propoxur and permethrin in ratsBackground: NMR combined with pattern recognition was recently introduced as a new technique for rapid xenobiotic toxicity evaluation. In this article, metabolic changes in the biofluid of rats after...Posted 10/01/2013 (4 months ago)
- A validated UHPLC–diode array detector method for the bioanalysis of atypical antipsychotics in whole blood, urine and cerebrospinal fluid following SPEBackground: Risperidone, quetiapine, amisulpride and aripiprazole are atypical antipsychotics increasingly prescribed in Greece. Results: We have developed and validated a UHPLC–diode array...Posted 10/01/2013 (4 months ago)
- Conference Report: Analytical chemistry in the Aegean Sea region: current statusThe Eighth Aegean Analytical Chemistry Days Conference took place in Urla, İzmir, Turkey, from 16–20 September 2012. This conference is held every 2 years, organized alternately by...Posted 08/01/2013 (4 months ago)
- Conference Report: Report on the 12th International Conference on Flow AnalysisThe 12th International Conference on Flow Analysis, referred to as ‘Flow Analysis XII’ was a 5-day scientific meeting that covered all recent advances in the field of flow analytical...Posted 08/01/2013 (4 months ago)
- Ontogenic stages for chemical instrumentationI am at the stage of life where organ systems draw on some of their redundancies. Repairs to physical insults take much longer now. I am hoping some wisdom has been gained, but one can never be sure....Posted 08/01/2013 (4 months ago)
- Investigation of basic mobile phases with positive ESI LC–MS for metabonomics studiesBackground: Accurate mass based LC–MS combined with statistical analysis is established as a core analytical technology for metabonomic studies. This is primarily due to the specificity,...Posted 20/12/2012 (5 months ago)
- Development and validation of an ultra-sensitive method for the measurement of plasma renin activity in human plasma via LC–MS/MSBackground: Renin catalyzes the conversion of angiotensinogen to angiotensin I (Ang I), the first and rate-limiting step in the renin–angiotensin–aldosterone system. Plasma renin activity...Posted 20/12/2012 (5 months ago)
- Conference Report: Drug Metabolism Discussion Group Short Meeting: microsampling – the next big thingOn behalf of the Drug Metabolism Discussion Group, Regulatory Bioanalysis AstraZeneca (UK) recently organized and hosted an extremely successful Drug Metabolism Discussion Group Short Meeting on...Posted 20/12/2012 (5 months ago)
- Conference Report: The 3rd EBF Focus Meeting: ‘Hatching’ – emerging technologies approaching the regulated bioanalysis laboratoryOne hundred and eighty scientists from industry and academia discussed the progress in emerging technologies approaching regulated bioanalysis, with a focus on what potential hurdles prevent them...Posted 20/12/2012 (5 months ago)
- Bringing new technologies into regulatory space “…collaboration between the bioanalytical scientific community and regulatory agencies is best positioned to enhance the overall benefits of new technologies and sustainably...Posted 20/12/2012 (5 months ago)
- Automation in new frontiers of bioanalysis: a key for quality and efficiency “To achieve high efficiency and quality in these new frontiers, automation is a key component and needs to be explored, evaluated and exploited.”
Posted 20/12/2012 (5 months ago)
- Polyphenols excreted in urine as biomarkers of total polyphenol intakeBackground: Nutritional biomarkers have several advantages in acquiring data for epidemiological and clinical studies over traditional dietary assessment tools, such as food frequency questionnaires....Posted 18/12/2012 (5 months ago)
- Direct bioanalytical sample injection with 2D LC–MSPosted 18/12/2012 (5 months ago)
- European Bioanalysis Forum recommendation: scientific validation of quantification by accelerator mass spectrometryAccelerator mass spectrometry (AMS) is being used more widely to provide PK data for early decision making or to generate absolute bioavailability data in later phases of development. Presently,...Posted 13/12/2012 (5 months ago)
- Revolutionizing SPE for improved bioanalysis“SPE is an indispensable tool for the bioanalyst and recent developments have allowed it to continue offering effective sample preparation solutions for modern laboratories.”
Posted 13/12/2012 (5 months ago)
- Biomarker assay development and validation for large molecules in bioanalysis: what are the challenges and how are they overcome?“..there is no substitute for experience, so as a community we must continue to commit to our collective education, regularly sharing our solutions, successes and failures through a variety of...Posted 13/12/2012 (5 months ago)
- Bioanalysis of drug in tissue: current status and challengesDistribution of drugs into tissues is an important determinant of the overall PK and PD profile. Thus, bioanalysis of drugs and their metabolites in tissues can play an important role in...Posted 06/12/2012 (5 months ago)
- Evaluation of surrogate matrices for standard curve preparation in tissue bioanalysisBackground: The aim of this work was to investigate the feasibility of using tissue surrogate matrices and plasma for determining drug concentrations in tissues by LC–MS/MS. Results: The...Posted 06/12/2012 (5 months ago)
- Tissue analysis – a powerful tool for drug discovery and development“In order to provide good quality data, we must understand that tissue analysis is no longer an endeavor of a single person or a single functional group.”
Posted 04/12/2012 (5 months ago)
- MALDI imaging MS analysis of drug distribution in tissue: the right time!(?)“The application of MALDI-IMS to determine the tissue distribution of drugs and their metabolites is the type of innovative technology that can have a dramatic impact on both drug discovery and...Posted 04/12/2012 (5 months ago)
- Conference Report: Emerging technology for bioanalysis in the next decadeThe 13th Annual University of Wisconsin Land O’Lakes Bioanalytical Conference
16–20 July 2012, Merrimac, WI, USA
This University of Wisconsin School of Pharmacy bioanalytical conference...Posted 04/12/2012 (5 months ago)
- Relative quantification of biomarkers using mixed-isotope labeling coupled with MSThe identification and quantification of important biomarkers is a critical first step in the elucidation of biological systems. Biomarkers take many forms as cellular responses to stimuli and can be...Posted 29/11/2012 (1 year ago)
- Prandial ghrelin attenuation provides evidence that des‑acyl ghrelin may be an artifact of sample handling in human plasmaBackground: Plasma acyl and des‑acyl ghrelin are thought of as components of total ghrelin, but this has never been validated using ex vivo spiking experiments, human sample collection...Posted 29/11/2012 (1 year ago)
- Early human ADME using microdoses and microtracers: bioanalytical considerationsQuantitative assessment of metabolites of drug candidates in early-phase clinical development presents an analytical challenge when methods, standards and assays are not yet available.
Posted 26/11/2012 (1 year ago)
- Analytical validation of accelerator mass spectrometry for pharmaceutical developmentThe validation parameters for pharmaceutical analyses were examined for the accelerator mass spectrometry measurement of 14C/C ratio, independent of chemical separation procedures.
Posted 26/11/2012 (1 year ago)
- Accelerator mass spectrometry best practices for accuracy and precision in bioanalytical 14C measurementsAccelerator mass spectrometers have an energy acceleration and charge exchange between mass definition stages to destroy molecular isobars and allow single ion counting of long-lived isotopes such as...Posted 26/11/2012 (1 year ago)
- Recommendations on biomarker bioanalytical method validation by GCCThe 5th GCC in Barcelona (Spain) and 6th GCC in San Antonio (TX, USA) events provided a unique opportunity for CRO leaders to openly share opinions and perspectives, and to agree upon recommendations...Posted 22/11/2012 (1 year ago)
- Important considerations for quantitation of small-molecule biomarkers using LC–MS“In our opinion, dealing with endogenous levels of the analyte, confirming specificity of the assay, maintaining analyte integrity ex vivo and designing the proper study protocol, comprise the...Posted 22/11/2012 (1 year ago)
- Bioanalysis of biomarkers for drug development“We hope the readers will benefit from the current thinking in the biomarker field while appreciating the diversity of challenges still facing the industry. This field is still evolving and we...Posted 22/11/2012 (1 year ago)
- Application of commercial research-grade biomarker assays in drug development: is it time to create ‘pharmaceutical-grade’ kits?“In pharmaceutical/biotechnology companies, the demand for increased innovation and efficiency has never been greater, but, at the same time, there is a profound need to reduce the overall cost...Posted 22/11/2012 (1 year ago)
- Interview with Mike Butler (Xceleron)In November 2012, Mike Butler (Xceleron) spoke to Alexandra Sklan at Bioanalysis Zone about Xceleron’s recent move to the USA, and the implications of this relocation on the development of...Posted 01/11/2012 (1 year ago)
- Split calibration curve: an approach to avoid repeat analysis of the samples exceeding ULOQBackground: The current practice of using calibration curves with narrow concentration ranges during bioanalysis of new chemical entities has some limitations and is time consuming. In the present...Posted 30/10/2012 (1 year ago)
- Surrogate matrix and surrogate analyte approaches for definitive quantitation of endogenous biomoleculesBackground: Quantitation of biomarkers by LC–MS/MS is complicated by the presence of endogenous analytes. This challenge is most commonly overcome by calibration using an authentic standard...Posted 30/10/2012 (1 year ago)
- Conference Report: 6th GCC focus on LBA: critical reagents, positive controls and reference standards; specificity for endogenous compounds; biomarkers; biosimilarsThe 6th Global CRO Council for Bioanalysis (GCC) Closed Forum was held on 27 March 2012 in San Antonio, TX, USA, the day before the start of the 6th Workshop on Recent Issues in Bioanalysis. The...Posted 25/10/2012 (1 year ago)
- Extraction and microextraction in bioanalysis “…the application of various liquid-phase extraction methods should be encouraged whenever possible, especially in combination with new analytical instruments…”
Posted 25/10/2012 (1 year ago)
- Measuring productivity in bioanalysis “While technical innovations have made the possibility of more rapid sample flow a reality, they have also made it possible to develop more robust methods in a timely manner.”
Posted 25/10/2012 (1 year ago)
- A workflow for the metabolomic/metabonomic investigation of exhaled breath using thermal desorption GC–MSBackground: Confounding factors in the analysis of human breath by thermal desorption GC–MS are reviewed, with special emphasis on the high water levels encountered in human breath samples....Posted 16/10/2012 (1 year ago)
- Application of metabolomics approaches to the study of respiratory diseasesMetabolomics is the global unbiased analysis of all the small-molecule metabolites within a biological system, under a given set of conditions. These methods offer the potential for a holistic...Posted 16/10/2012 (1 year ago)
Performance Tested LC–MS Ultra CHROMASOLV® SolventsPosted 15/10/2012 (1 year ago)- Realising the potential of metabolomics “…metabolomics is starting on its next golden age; one in which it will finally emerge and be recognized for the significant benefit it can provide.”
Posted 11/10/2012 (1 year ago)
- 2012 White Paper on Recent Issues in Bioanalysis and Alignment of Multiple GuidelinesOver 400 professionals representing pharmaceutical companies, CROs, and multiple regulatory agencies participated in the 6th Workshop on Recent Issues in Bioanalysis (WRIB). Like the previous...Posted 11/10/2012 (1 year ago)
- The role of metabolomics in characterizing the human exposome “…[the exposome concept] represents a paradigm shift in molecular epidemiology/environmental toxicology, with the focus on complex human exposure profiles (‘top-down’),...Posted 11/10/2012 (1 year ago)
- Maximizing the value of metabolomic data “Untargeted metabolomic data are exceedingly rich in information and, consequently, researchers typically focus on only a small subset of the results. The remaining un-mined portions of...Posted 11/10/2012 (1 year ago)
- Interview with Tom Astle (Tomtec) – the effect of hematocrit in DBS studiesTom Astle (Tomtec; CT, USA) speaks to Alexandra Sklan at Bioanalysis Zone in October 2012 about the variation in the results generated in two recent hematocrit DBS analytical studies. This interview...Posted 11/10/2012 (1 year ago)
- Immunological screening and characterization of highly specific monoclonal antibodies against 20 kDa hGHBackground: hGH has been widely abused as a doping agent in sports for many years. There are some important approaches for the detection of hGH doping, and the ratio of 22:20 kDa GH was considered...Posted 04/10/2012 (1 year ago)
- Simultaneous quantification of davalintide, a novel amylin-mimetic peptide, and its active metabolite in beagle and rat plasma by online SPE and LC–MS/MSBackground: Davalintide, an investigational therapeutic peptide for the treatment of obesity, is rapidly metabolized by enzymatic cleavage of its N-terminal lysine residue to produce an active...Posted 04/10/2012 (1 year ago)
- Recommendations on bioanalytical method stability implications of co-administered and co-formulated drugs by Global CRO Council for Bioanalysis (GCC)An open letter written by the Global CRO Council for Bioanalysis (GCC) describing the GCC survey results on stability data from co-administered and co-formulated drugs was sent to multiple regulatory...Posted 03/10/2012 (1 year ago)
- Protein quantification using LC–MS: can it make a difference? “LC–MS can certainly add value for protein quantification, in particular because of its reliable nature and the resulting improved comparability of results between...Posted 02/10/2012 (1 year ago)
- Quantitation by MS imaging: needs and challenges in pharmaceuticals “MS imaging, which is particularly useful in ophthalmology, neurology, dermatology, renal or hepatotoxicity, is not limited to any therapeutic area.”
Posted 02/10/2012 (1 year ago)
- Determination of drugs of abuse in hair “Hair has been focused on for its usability as an alternative biological specimen to blood and urine for determining drugs of abuse in fields ... because hair can be used to elucidate the...Posted 02/10/2012 (1 year ago)
- A toxicological perspective on the use of spin column extraction to detect drugs and metabolites in biological matrices “...commercially available columns or devices for monoliths are still limited compared with SPME. In the future, monoliths are expected to be used for the extraction and purification of a...Posted 02/10/2012 (1 year ago)
Utilization of hydrophilic-interaction LC to minimize matrix effects caused by phospholipidsBackground: In bioanalysis, phospholipids may affect the precision and accuracy of LC–MS/MS methods and compromise the quality of the results, especially when samples in complex biomatrices are...Posted 13/09/2012 (1 year ago)
Strategies to estimate and improve drug tolerance in anti-drug antibody assaysBackground: Drug tolerance of anti-drug antibody (ADA) assays is becoming increasingly important due to the high number of newly emerging long-acting drugs. Methods to estimate and improve drug...Posted 13/09/2012 (1 year ago)- The potential application of electromembrane extraction for the analysis of peptides in biological fluids“...EME is compatible with different biological fluids, including human plasma and whole blood, which are relevant samples for peptide analysis. Therefore, it is highly relevant to investigate...Posted 12/09/2012 (1 year ago)
- Updates from the EBF DBS–microsampling consortium“...the consortium agreed to work on four aspects of DBS, which were highlighted in the EBF Recommendation paper as potentially being a hurdle towards applicability and implementation of the...Posted 11/09/2012 (1 year ago)
- European Bioanalysis Forum recommendation on method establishment and bioanalysis of biomarkers in support of drug developmentBiomarkers have become increasingly important in drug development and many bioanalysts are getting involved. Consequently, different views on how to approach the bioanalysis of biomarkers have been...Posted 10/09/2012 (1 year ago)
Challenges faced by the modern bioanalytical laboratoryTim Sangster (Charles River Laboratories) and Mike Oliver (Thermo Fisher Scientific) speak to Thomas Payne at Bioanalysis Zone in September 2012 about the challenges faced by the modern bioanalytical...Posted 24/10/2012 (1 year ago)
- Applications of accelerator MS in pediatric drug evaluationAccelerator MS (AMS) provides a novel method for obtaining and analyzing pharmacokinetics and pharmacodynamics in children. This paper reviews the scientific and ethical rationale for AMS in...Posted 06/09/2012 (1 year ago)
Overcoming bioanalytical challenges in an Onglyza® intravenous [14C]microdose absolute bioavailability study with accelerator MSBackground: An absolute bioavailability study that utilized an intravenous [14C]microdose was conducted for saxagliptin (Onglyza®), a marketed drug product for the treatment of Type 2 diabetes...Posted 06/09/2012 (1 year ago)- Measurement and validation of cell-based assays with microfluidics at the National Institute of Standards and TechnologyThe National Institute of Standards and Technology (NIST) is the National Metrology Institute for the USA. Our mission is to advance measurement science, standards and technology in ways that enhance...Posted 05/09/2012 (1 year ago)
- Conference Report: 3rd Annual Symposium of Chemical and Pharmaceutical Structure AnalysisThe 3rd Annual Symposium on Chemical and Pharmaceutical Structure Analysis was once again held in Shanghai, where a rich history of ‘East meets West’ continued. This meeting is dedicated...Posted 05/09/2012 (1 year ago)
- Could traditional mass-balance studies in the ‘twilight zone’ be improved by means of accelerator MS measurements?“…when accelerated MS becomes a common and commercially available technique, it will no longer be restricted to microdose studies. Rather, it will contribute to and have a role in 14C...Posted 05/09/2012 (1 year ago)
- When opportunity met aspirational goals: accelerator MS, microdosing and absolute bioavailability studies“…the pharmaceutical industry became quite adept at deriving an estimate of absolute bioavailability without necessarily developing an intravenous formulation and performing the...Posted 05/09/2012 (1 year ago)
- The use of selected reaction monitoring in quantitative proteomicsSelected reaction monitoring (SRM) has a long history of use in the area of quantitative MS. In recent years, the approach has seen increased application to quantitative proteomics, facilitating...Posted 15/08/2012 (1 year ago)
- Quantitative/qualitative analysis using LC–HRMS: the fundamental step forward for clinical laboratories and clinical practice“Clinical LC–MS analysis employing HRMS acquisitions for routine Quan/Qual analysis is on the horizon and the future will bring real progress built on the same foundation: targeted...Posted 14/08/2012 (1 year ago)
- The next big thing is actually smallRecent developments in materials, surface modifications, separation schemes, detection systems and associated instrumentation have allowed significant advances in the performance of lab-on-a-chip...Posted 09/08/2012 (1 year ago)
- Improve LC-MS Run Time/Performance & Reduce Risk of Ion-SuppressionPosted 08/08/2012 (1 year ago)
- Comparison of Sample Prep TechniquesPosted 08/08/2012 (1 year ago)
- Ion-Suppression & Phospholipid ContaminationPosted 08/08/2012 (1 year ago)
- Fast HPLC Using Fused-Core Particle Technology for Rapid Screening of Pharmaceutical CompoundsPosted 08/08/2012 (1 year ago)
- Vitamin D and Prevention of Human DiseasePosted 08/08/2012 (1 year ago)
- Synthetic Cannabinoid Analysis – Standards & MethodsPosted 08/08/2012 (1 year ago)
- Increase Bioanalytical Assay Speed via Phospholipid Removal using HybridSPE™-Phospholipid TechnologyPosted 08/08/2012 (1 year ago)
- Minimizing Phospholipid Matrix Effects in HILIC LC-MSPosted 08/08/2012 (1 year ago)
- Increased Bioanalytical Throughput and Recovery Using HybridSPE-PLPosted 08/08/2012 (1 year ago)
- Enrichment of Phospholipids in Biological Samples Using HybridSPE-PLPosted 08/08/2012 (1 year ago)
- Metabolomic profiling of Neurospora crassa fungi using HILIC and reversed phase LC–MSPosted 08/08/2012 (1 year ago)
- Recommendations on ISR in multi‑analyte assays, QA/bioanalytical consultants and GCP by Global CRO Council for Bioanalysis (GCC)The 5th Global CRO Council for Bioanalysis (GCC) meeting, held in Barcelona, Spain, in November 2011, provided a unique opportunity for CRO leaders to openly share opinions, perspectives and to agree...Posted 08/08/2012 (1 year ago)
- Supel-Select HLB SPE for Pharmaceutical AnalysisPosted 07/08/2012 (1 year ago)
- SPME in the Analysis of Illicit DrugsPosted 07/08/2012 (1 year ago)
Is the bioanalytical community ready to revise the use of certain validation concepts and nomenclature? “Having a harmonized definition of what defines a method and with the differentiation of the validation experiments, we believe it is possible to improve clarity in the regulatory...Posted 07/08/2012 (1 year ago)- Assessment of the Fatty Acid Status in Blood LipidsPosted 06/08/2012 (1 year ago)
- Accelerated Bioanalytical LC-MS-MS | Ascentis® ExpressPosted 07/08/2012 (1 year ago)
- Serum Lipidomics Profiling using an Ascentis Express Fused-Core C18 ColumnPosted 07/08/2012 (1 year ago)
- Serum Lipidomics Profiling using an Ascentis Express Fused-Core C18 ColumnPosted 07/08/2012 (1 year ago)
- Metabolites – A Serious Challenge for LC/MS Separations and Usual Detection TechniquesPosted 07/08/2012 (1 year ago)
- Derivatization Agents for LC/MS – An Improved Detection of Estradiol with ESI-MSPosted 07/08/2012 (1 year ago)
- Fast and Accurate LC-MS Analysis of Vitamin D Metabolites Using Ascentis® Express F5 HPLC ColumnsPosted 07/08/2012 (1 year ago)
- Fast LC-MS-MS Analysis of 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3lPosted 07/08/2012 (1 year ago)
- Vitamin D epi-Metabolites Separated with HybridSPE-PhospholipidPosted 07/08/2012 (1 year ago)
- Alternative Retention and Selectivity Using Fluorinated Stationary PhasesPosted 07/08/2012 (1 year ago)
- Bioanalysis with SPMEPosted 07/08/2012 (1 year ago)
- Molecularly Imprinted Polymer SPE- Acid NSAIDs from MusclePosted 06/08/2012 (1 year ago)
- Derivatization and Separation of Aliphatic AminesPosted 06/08/2012 (1 year ago)
- Benzodiazepines on Ascentis® Express Phenyl Hexyl HPLC ColumnsCombining the popular Phenyl-Hexyl Stationary Phase and Fused-Core® Particles
Posted 06/08/2012 (1 year ago)
- How to Overcome Suppression Effects of TFALC-MS Mobile Phase Additives – Tips & Tricks
Posted 06/08/2012 (1 year ago)
- Maximize Speed and Sensitivity of Bioanalytical AssaysIt is truly an Analytical World we serve. Our customers are located in all countries of the world.They work in industry, government and academia. They are involved in manufacturing, quality...Posted 06/08/2012 (1 year ago)
- Ethical considerations in anti-doping analysis“Everyone who participates in the effort to deter cheating, through education, research or the process of collection, testing and adjudication, is in a position to support honorable athletes in...Posted 03/08/2012 (1 year ago)
- Half a century of challenges“The appearance of new potential doping substances has often been accompanied by comments suggesting that they would be impossible to detect at doping controls … And now we hear the same...Posted 02/08/2012 (1 year ago)
- Accelerated Bioanalytical LC-MS-MS | Ascentis® ExpressFast bioanalysis requires the use of short columns packed with high efficiency particles. The use of HPLC columns packed with Fused-Core particles and sub-2 μm porous particles have led the way in...Posted 01/08/2012 (1 year ago)
- Current limitations in analytical strategies“Clearly, there always will be athletes who want to dope … But the overall strategy for the fight against doping in sport has undoubtedly become more coherent in recent years and...Posted 01/08/2012 (1 year ago)
- Interview: Partnering with the industry: the Olympic laboratory modelDavid A Cowan and Campbell P Barker speak to Ryan De Vooght-Johnson at Bioanalysis in May 2012 about the partnership between industry and academia for the setup and running of the doping-control...Posted 31/07/2012 (1 year ago)
- Anti-doping analysis and the Olympics“The fight against doping, unlike medical practice, must work with an infinitesimal margin of uncertainty.”
Posted 30/07/2012 (1 year ago)
- Perspectives on microsampling: DBSThe concept of microsampling and particularly the dried blood spot methodology has been widely known to the scientific community for considerable time. Yet, there is no formal standard approach...Posted 26/07/2012 (1 year ago)
2012 Bioanalysis Young Investigator: Maria Lorna A De LeozMaria Lorna A De Leoz
National Institute of Standards & Technology, 100 Bureau Drive, Stop 8362 Gaithersburg, MD 20899, USA
Michael J Tarlov
Deputy Chief, Biochemical Science Division,...Posted 24/07/2012 (1 year ago)
- 2012 Bioanalysis Young Investigator: Matthew BlatnikMatthew Blatnik
Position: Senior Scientist; PhD: Biochemistry; Research area: Translational Biomarker Research; Pfizer Inc., 1 Eastern Point Rd, Groton, CT 06340, USA.
Xiaogang Han
Pfizer...Posted 24/07/2012 (1 year ago)
- Semi-automated direct elution of dried blood spots for the quantitative determination of guanfacine in human bloodBackground: Direct analysis of dried blood spot (DBS) samples was investigated using a prototype semi-automated robotic device that allows the direct elution of sample spots from a DBS paper card to...Posted 19/07/2012 (1 year ago)
- Your bioanalytical data are only as good as your samples“Good data can only come from good samples. Technology is now available to collect, transport, store and document samples like never before. It is critical that these new tools be given as much...Posted 18/07/2012 (1 year ago)
Meeting the demands of regulatory requirements: the significance of ADME“ADME will continue to play an active and expanding role in drug discovery and development, particularly in helping improve the accuracy and precision of PK/PD model predictions...”
Posted 16/07/2012 (1 year ago)- High-field asymmetric waveform ion mobility spectrometry with solvent vapor addition: a potential greener bioanalytical techniqueGreen chemistry is a way to avoid threats to human health and the environment in chemical processes, including analytical methodology. According to the 12 principles provided by ACS Green Chemistry...Posted 11/07/2012 (1 year ago)
- The benefits of using solid-phase microextraction as a greener sample preparation technique“[In vivo solid-phase microextraction sampling] can ensure not only preservation of the analyte and protection from its degradation during sampling and extraction, which is particularly...Posted 09/07/2012 (1 year ago)
- 2012 Bioanalysis Young Investigator: Raphael Bastos Mareschi AggioRaphael Bastos Mareschi Aggio
Position: PhD student; PhD: Bioinformatics and Systems Biology; Research area: Bioinformatics and Systems Biology; The University of Auckland, 12 Godfrey Place,...Posted 05/07/2012 (1 year ago)
- 2012 Bioanalysis Young Investigator: Omnia A IsmaielOmnia A Ismaiel
Position: Research Scientist and Lecturer of Analytical Chemistry; PhD: Analytical Chemistry; Research area: Bioanalytical MS; Chromatographic Science Department, PPD, 2244 Dabney...Posted 05/07/2012 (1 year ago)
- Green bioanalytical methods are now a reality“...it is clear from the articles in this issue that the interest in green bioanalytical methods is growing in the drug-discovery and -development field.”
Posted 02/07/2012 (1 year ago)
- Green bioanalytical chemistry“…analytical chemistry is an information science that need not consume more resources than are necessary to support the analysis.”
Posted 29/06/2012 (1 year ago)
MARS: bringing the automation of small-molecule bioanalytical sample preparations to a new frontier Background: In recent years, there has been a growing interest in automating small-molecule bioanalytical sample preparations specifically using the Hamilton MicroLab® STAR liquid-handling...Posted 26/06/2012 (1 year ago)- Will ‘green’ aspects of dried blood spot sampling accelerate its implementation and acceptance in the pharmaceutical industry?"The broader application of DBS in pharmacokinetic analysis is an excellent example of a ‘green’ technique based on the benefits in reduction of animals, chemicals, labware and...Posted 26/06/2012 (1 year ago)
- Biomarker monitoring in sports doping controlBiomarker monitoring can be considered a new era in the effort against doping. Opposed to the old concept in doping control of direct detection of a prohibited substance in a biological sample such...Posted 20/06/2012 (1 year ago)
- Validation study of urinary metabolites as potential biomarkers for prostate cancer detectionBackground: Urinary metabolomic profiles have recently drawn a lot of attention owing to a debate regarding their possible role as potential clinical markers for prostate cancer. In this study,...Posted 18/06/2012 (1 year ago)
- 2012 Bioanalysis Young Investigator: Maria Rambla-AlegreMaria Rambla-Alegre
Position: Post-Doctoral Researcher; PhD: Analytical Chemistry; Research area: Seperation Science, Organic Chemistry Department; Ghent University, Ghent, Belgium Brabantdam 117C,...Posted 12/06/2012 (1 year ago)
- 2012 Bioanalysis Young Investigator: Dajana VuckovicDajana Vuckovic
Position: Postdoctoral Fellow; PhD: Analytical Chemistry; Research area: Bioanalytical Chemistry, Metabolomics and Proteomics; Donnelly Centre for Cellular & Biomolecular...Posted 12/06/2012 (1 year ago)
- Conference Report: Bioanalytical sessions at the 2011 Eastern Analytical SymposiumThe 2011 Eastern Analytical Symposium (EAS) and Exposition was held at the Garden State Exhibit Center in Somerset NJ, USA, 14–17 November and marked the 50th anniversary of EAS, with a theme...Posted 06/06/2012 (1 year ago)
- Biomarkers and biomeasures: key enablers for pharmacokinetic–pharmacodynamic modeling in drug discovery and development"While biomarkers quantitatively measure perturbations to the system anywhere in the causal path between drug administration and clinical effect … biomeasures are quantitative information...Posted 05/06/2012 (1 year ago)
- Recent development in software and automation tools for high-throughput discovery bioanalysisBioanalysis with LC–MS/MS has been established as the method of choice for quantitative determination of drug candidates in biological matrices in drug discovery and development. The...Posted 04/06/2012 (1 year ago)
- Conference Report: Isranalytica 2012The Isranalytica, organized by the Israel Analytical Chemical Society, is the largest analytical conference in Israel and has become one of the largest annual conferences in analytical chemistry in...Posted 01/06/2012 (1 year ago)
- Discovery pharmacokinetic studies in mice using serial microsampling, dried blood spots and microbore LC–MS/MSBackground: Initial pharmacokinetic (PK) studies of discovery compounds are conducted in mice to demonstrate exposure prior to conducting efficacy studies. PK information obtained from a single mouse...Posted 31/05/2012 (1 year ago)
- 2012 Bioanalysis Young Investigator: Pan DengPan Deng
Position: Research assistant; PhD: Pharmaceutical analysis; Research area: Drug metabolism and pharmacokinetics; Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Haike...Posted 29/05/2012 (1 year ago)
- 2012 Bioanalysis Young Investigator: Sathyadevi VenkataramaniSathyadevi Venkataramani
Position: Scientist I; PhD: Biochemistry/biophysics; Research area: Protein analytical characterization; Integrated BioTherapeutics, 21 Firstfield Road, Gaithersburg, MD...Posted 29/05/2012 (1 year ago)
- Simultaneous qualitative and quantitative metabolism data generation: a convenient marriage?“For the [qual/quan] concept to truly revolutionize drug discovery, a way must be found to successfully combine the expertise encompassed within the biotransformation and high-throughput...Posted 24/05/2012 (1 year ago)
- Discovery biotherapeutics bioanalysis: challenges and possible solutions“Discovery bioanalysis requires a balance between speed, cost savings and analytical experience to ensure that results can be interpreted accurately with appropriate caveats to allow projects...Posted 24/05/2012 (1 year ago)
- Surrendering to the robot army: why we resist automation in drug discovery and development“Generals in the robot army should brace themselves with a healthy dose of intestinal fortitude, but the long-term benefits will far outweigh the pain.”
Posted 22/05/2012 (1 year ago)
- Discovery bioanalysis“Discovery bioanalysis is a very technology-driven field and … the motivation to constantly ‘push the envelope’, and the willingness to evaluate and adopt novel MS-based and...Posted 21/05/2012 (1 year ago)
- 2012 Bioanalysis Young Investigator: Eugene CiccimaroEugene Ciccimaro
Position: Applications Specialist; PhD: Pharmacology; Research area: Quantitative Proteomics and Metabolomics; Thermo Scientific, 265 Davidson Ave, Somerset, NJ 08873, USA.
Ian A...Posted 15/05/2012 (1 year ago)
- 2012 Bioanalysis Young Investigator: Michael GroesslMichael Groessl
Position: Research Associate; PhD: Chemistry; Research area: Bioanalytical Chemistry; Max Planck Institute of Molecular Cell Biology & Genetics, Pfotenhauerstr. 108, 01307...Posted 15/05/2012 (1 year ago)
- Recent advances in metabolite identification and quantitative bioanalysis by LC–Q-TOF MSThe need for rapid, sensitive and effective identification and quantitation of drugs and metabolites to accelerate drug discovery and development has given MS its central position in drug metabolism...Posted 09/05/2012 (1 year ago)
- Development of a method by UPLC–MS/MS for the quantification of tizoxanide in human plasma and its pharmacokinetic applicationBackground: Nitazoxanide (NTZ) is used for the treatment of gastrointestinal tract colonization by anaerobic bacteria, viruses and other pathogens that represent a major cause of morbidity in Latin...Posted 08/05/2012 (1 year ago)
- Bioanalysis of biotherapeutic proteins and peptides: immunological or MS approach?Historically, the bioanalysis of proteins and peptides has been performed using immunological-based techniques. However, continued improvements in MS-based methodologies over the past 10 years have...Posted 04/05/2012 (1 year ago)
- European Medicines Agency guideline on bioanalytical method validation: what more is there to say?The European Medicines Agency (EMA) published a draft guideline on bioanalytical method validation in November 2009 (hereafter referred to as the ‘Guideline’) and following a consultation...Posted 02/05/2012 (1 year ago)
- Fast chromatography in the regulated bioanalytical environment: sub-2-µm versus fused-core particlesSeveral companies have recently experienced, or will soon experience, patent expirations that will hurt profits and are looking for faster and cheaper ways to develop their pipelines to compensate...Posted 30/04/2012 (1 year ago)
- Recent advances in column switching sample preparation in bioanalysisColumn switching techniques, using two or more stationary phase columns, are useful for trace enrichment and online automated sample preparation. Target fractions from the first column are...Posted 27/04/2012 (1 year ago)
- Challenges in developing an ultra-sensitive bioanalytical method for ethinylestradiol in human plasmaBackground: Ethinylestradiol (EE) is the active component in most birth control products. It is especially difficult to analyze due to the presence of many closely related endogenous steroids....Posted 25/04/2012 (1 year ago)
- New methodology for the development of chromatographic methods with bioanalytical applicationBioanalytical methods are developed in order to answer the analytical problems faced by analysts. The analytical issues that must be solved are generally the detection and/or quantification of an...Posted 23/04/2012 (1 year ago)
- Conference Report: Advances in Metabolic ProfilingThe main topics of this conference were based around new technologies and applications of metabolomics in healthcare, bioscience and environmental applications. There were sessions themed on...Posted 18/04/2012 (1 year ago)
- Recent advances in liquid microextraction techniques coupled with MS for determination of small-molecule drugs in biological samplesSample preparation is an important and necessary step in a measurement process for isolation and concentration of desired components from complex matrices. It is the most time-consuming and...Posted 16/04/2012 (1 year ago)
- Quantitation of polypeptides (glucagon and salmon calcitonin) in plasma samples by ‘high resolution’ on a triple quadrupole mass spectrometerBackground: Bioanalysis of large molecules can be challenging on LC–MS/MS due to multiply charged and endogenous interferences. While high sensitivity triple quadrupole systems (QqQ) have been...Posted 12/04/2012 (1 year ago)
- Letter to the Editor: Response to: Commentary on the history and quantitative nature of filter paper used in blood collection devicesPosted 10/04/2012 (1 year ago)
- Letter to the Editor: Commentary on the history and quantitative nature of filter paper used in blood collection devicesIt is with great interest that we read Peter T Kissinger’s commentary about the use of dried blood spots (DBS) for pharmacokinetic assays and therapeutic drug monitoring published in the...Posted 10/04/2012 (1 year ago)
- Combining label-free technologies: discovery in strengthSince the early 1990s when Biacore entered the market with the introduction of surface plasmon resonance (SPR) for use in elucidating interactions of proteins, label-free technologies have gained...Posted 06/04/2012 (1 year ago)
- Recommendations on the interpretation of the new European Medicines Agency Guideline on Bioanalytical Method Validation by Global CRO Council for Bioanalysis (GCC)Senior representatives of GCC member companies have thoroughly evaluated and discussed the new European Medicines Agency (EMA; July 2011 [1]) Guideline on Bioanalytical Method Validation (BMV),...Posted 03/04/2012 (1 year ago)
- Conference Report: ‘Less is More’: defining modern bioanalysisThe 4th Open Symposium of the European Bioanalytical Forum entitled ‘Less is More’ was held on 16–18 November 2011 at the Hesperia Tower Hotel, Barcelona, Spain. More than 50...Posted 30/03/2012 (1 year ago)
- Conference Report: ‘Large Meets Small’: connecting the bioanalytical community around peptide and protein bioanalysis with LC–MS(/MS)This conference report provides an overview of the discussions at the 2nd European Bioanalysis Forum (EBF) Focus Meeting ‘Large Meets Small’ held on 20 and 21 June 2011 in Brussels. The...Posted 29/03/2012 (1 year ago)
- Interview with Christophe P. Stove (Ghent University) – DBS in toxicologyThe Laboratory of Toxicology research group at the Faculty of Pharmaceutical Sciences of Ghent University (Ghent, Belgium) have recently published a paper providing an overview of the different DBS...Posted 27/03/2012 (1 year ago)
- Webinar: Bioanalysis of Therapeutic and Endogenous Peptides: Understanding and Overcoming the Challenges of Working with Large MoleculesPosted 22/03/2012 (1 year ago)
- HRMS: current usage, future directions and the promise of integration with unified data streams suited to post-acquisition miningModern TOF and Orbitrap-based mass analyzers have made the acquisition of LC–high resolution MS (HRMS) datasets routine. These datasets are uniquely suited for combined...Posted 21/03/2012 (1 year ago)
- Conference Report: High-resolution MS in drug discovery and development: current applications and future perspectivesThe evolving use of high-resolution MS (HRMS) across the pharmaceutical industry has seen its integrated application into both quantitative and qualitative assays. Through an American Association of...Posted 19/03/2012 (1 year ago)
- The emergence of high-resolution MS as the premier analytical tool in the pharmaceutical bioanalysis arenaToday, high-resolution MS (HRMS) is used in many areas of science including the pharmaceutical industry. Looking back 100 years, a mass spectrometer with a mass resolving power of less than 20 was...Posted 15/03/2012 (1 year ago)
- Conference Report: Science and technology coming together to make a differenceThis annual meeting began in 1998 and was the first industry-led event to focus on the specific needs of industry researchers. The goal of Chemical and Pharmaceutical Structure Analysis is to provide...Posted 13/03/2012 (1 year ago)
- Towards single-cell analysis for pharmacokineticsTraditional ‘macroscopic’ pharmacokinetics (PK) investigates the fate of drugs or toxicants administered externally to living organisms, described by the extent and rate of absorption,...Posted 12/03/2012 (1 year ago)
- Managing the effect of hematocrit on DBS analysis in a regulated environmentThe majority of preclinical and clinical pharmacokinetic studies in pharmaceutical development are performed under Good Laboratory Practice regulations, ensuring the accuracy, reproducibility and...Posted 07/03/2012 (1 year ago)
- Evaluation of 5-methyl-2´-deoxycytidine stability in hydrolyzed and nonhydrolyzed DNA by HPLC–UVBackground: Although the determination of 5-methyl-2´-deoxycytidine (5-MedC) in various biological samples is gaining increasing scientific interest, there are no data available regarding its...Posted 05/03/2012 (1 year ago)
- In vitro evaluation of the potential for drug-induced toxicity based on 35S-labeled glutathione adduct formation and daily doseBackground: Drug-induced toxicity such as idiosyncratic drug toxicity is believed to be reduced when either reactive metabolite formation or exposure to a drug is minimized. The objective of the...Posted 20/02/2012 (1 year ago)
The most convenient and general approach for plasma sample clean-up: multifunction adsorption and supported liquid extractionThe vast number and increased diversity of molecular entities of drug candidates present new challenges to methodologies of bioanalysis in the process of drug discovery. Blood plasma represents a...Posted 13/02/2012 (1 year ago)- Conference Report: US FDA/EMA harmonization of their bioanalytical guidance/guideline and activities of the Global Bioanalytical ConsortiumThe 2011 annual conference of the American Association of Pharmaceutical Scientists, held in Washington DC, USA, hosted a roundtable entitled: ‘Update of the US FDA/European Medicines Agency...Posted 09/02/2012 (1 year ago)
- Methods for the analysis of nonbenzodiazepine hypnotic drugs in biological matricesZopiclone, zolpidem and zaleplon (Z-drugs) are nonbenzodiazepine hypnotic drugs that are used for the treatment of insomnia. These drugs were developed with the intent to overcome some disadvantages...Posted 06/02/2012 (1 year ago)
- Readers' Choice - vote for the best of 2011Help us select the best of Bioanalysis from 2011 - the winning article will be made freely available on Bioanalysis Zone.
Posted 01/02/2012 (1 year ago)
- Determination of lumefantrine in small-volume human plasma by LC–MS/MS: using a deuterated lumefantrine to overcome matrix effect and ionization saturationBackground: To support pediatric study, a method to determine lumefantrine (LF) with small sample volume is needed. Matrix effect (ME) is a daunting issue in LF quantification in human plasma with...Posted 30/01/2012 (1 year ago)
- Chemical derivatization as a tool for optimizing MS response in sensitive LC–MS/MS bioanalysis and its role in pharmacokinetic studiesChemical derivatization is the only technique that directly affects the physicochemical property of an analyte in an LC–MS/MS assay platform. On the other side, current MS instruments are...Posted 25/01/2012 (1 year ago)
- Bioanalytical method development: considering information from a suite of complementary methodsBioanalytical method development can be viewed as a science, an art, a fundamental part of the high-stakes game of pharmaceutical development or an admixture of the above. Specifications can change...Posted 23/01/2012 (1 year ago)
- A validated micellar LC method for simultaneous determination of furosemide, metoprolol and verapamil in human plasmaAn accurate and precise micellar LC method coupled with UV and fluorimetric detectors was developed and validated for the simultaneous analysis of furosemide, metoprolol and verapamil in human...Posted 18/01/2012 (1 year ago)
- An indecent proposal or improved quality of bioanalytical data in clinical studiesSince the late 1970s and early 1980s, technology in bioanalysis has developed tremendously. I think the most obvious improvement is the increase in sample analysis throughput. However, my aim in this...Posted 17/01/2012 (1 year ago)
- Conference Report: 40th Anniversary Drug Metabolism Discussion Group Open MeetingThe Drug Metabolism Discussion Group is an informal Europe-wide association for scientists engaged in research and development within drug metabolism, pharmacokinetics and other related disciplines...Posted 16/01/2012 (1 year ago)
- Conference Report: Boston Society’s 7th Applied Pharmaceutical AnalysisThe Boston Society hosted the 7th Applied Pharmaceutical Analysis (APA) conference in Cambridge (MA, USA) from September 12th to 14th 2011. The overarching theme for this year’s meeting was...Posted 13/01/2012 (1 year ago)
- Impact of the emergence of designer drugs upon sports doping testingHistorically, dope-testing methods have been developed to target specific and known threats to the integrity of sport. Traditionally, the source of new analytical targets for which testing was...Posted 11/01/2012 (1 year ago)
- Sound science versus anxiety: validation of bioanalytical assays for small moleculesWhen we report final bioanalytical quantitative data we occasionally feel some degree of anxiety. Are our data correct? Have the guidelines criteria from authorities been fulfilled and did we follow...Posted 09/01/2012 (1 year ago)
- Are multimetabolite methods fit-for-purpose for supporting toxicology studies?The measurement of parent drug in biological samples is an integral part of the drug-development process from discovery support through to late phase clinical development. At some point during this...Posted 09/01/2012 (1 year ago)
- The use of accelerator MS in support of MISTThe issue of how best to underwrite the safety of metabolites formed in humans has been an important consideration within the pharmaceutical industry for many decades. Following the publication, in...Posted 05/01/2012 (1 year ago)
- Bioanalysis through the agesThis article highlights many of the issues of concern for analytical scientists working in the area of bioanalysis. It draws mainly on topics presented at the Reid International Bioanalytical Forum,...Posted 04/01/2012 (1 year ago)
- Conference Report: The 19th International Reid Bioanalytical ForumThe 19th International Reid Bioanalytical Forum was attended by over 120 participants. The Forum divided into approximately eight broad topics, although not always in the same session. The meeting...Posted 04/01/2012 (1 year ago)
- Use of dried blood spots to study excipient kinetics in neonatesLiquid pharmaceutical formulations for parenteral and enteral use generally contain the active pharmaceutical ingredient (API, also known as the ‘drug’) and a range of other chemicals....Posted 03/01/2012 (1 year ago)
