A validated UHPLC–diode array detector method for the bioanalysis of atypical antipsychotics in whole blood, urine and cerebrospinal fluid following SPE

Background: Risperidone, quetiapine, amisulpride and aripiprazole are atypical antipsychotics increasingly prescribed in Greece. Results: We have developed and validated a UHPLC–diode array detector method for the determination of these drugs in whole blood, urine and cerebrospinal fluid, following SPE. The separation was achieved within 7 min. Validation was performed in terms of linearity, selectivity, accuracy, precision and stability. Following validation, the method was applied to post-mortem blood, urine and cerebrospinal fluid samples. Conclusion: The method proved fast and simple for the determination of four atypical antipsychotic drugs in whole blood, urine and cerebrospinal fluid. To the best of our knowledge,...