Bioanalysis Zone

Meeting the LC-MS needs of a high-throughput clinical laboratory


The sudden expansion of LC-MS/MS use within clinical laboratories has presented many challenges for hospital service providers and hardware & consumable vendors alike. Often the sample workflow and the types of target analytes measured differ greatly to those found in bio-analytical and pharmaceutical industries and thus sometimes require different strategies.

With examples from endocrinology and biochemical genetics unit disciplines within healthcare laboratories, this presentation will cover alternative sample extraction and liquid chromatography solutions developed to meet the challenges of a busy hospital laboratory.

Topics Covered:

  • Multiplexing on-line SPE to meet the needs of a high through-put clinical laboratory
  • Accelerating patient sample run times and providing system robustness by using superficially porous particle HPLC columns
  • LC-MS/MS Analysis of steroids, thyroid hormones, 25OH Vitamin D2/3 (and their epimers), metanephrines, 5HIAA, creatinine, creatine, guanidinoacetate and markers for lysosomal storage disorders
  • Selecting the appropriate column chemistry for target analytes
  • Use of phospholipid removal filter plates on human serum

Who attended:

  • Healthcare Scientists
  • Medics
  • HPLC and LC-MS users
  • Bio-analytical scientists


Mr Michael J.P. Wright

Mike Wright is an analytical chemist employed as the lead on HPLC and LC-MS/MS method development for Cambridge University Hospital Trust. In December 2009 he was appointed co-ordinator of the British Mass Spectrometry Society’s (BMSS) Clinical and Forensic Group with membership comprised of scientists from bio-analytical facilities, the pharmaceutical industry, academia and hospitals. Among his research interests include the possible applications of emerging sample preparation and separation techniques along with the quantitative LC-MS/MS measurement of sugars, bile acids, steroids and insulin.


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