Publication / Source: Bioanalysis 3(24)
Authors: Michopoulos F, Theodoridis G, Smith CJ, Wilson ID
Background: Investigations have been undertaken of the effects of storage on the utility of using dried blood spots, collected and stored on a paper substrate, combined with reversed-phase ultra-performance LC (UPLC), electrospray ionization orthogonal acceleration ToF-MS (UPLC–oaToF-MS), as a method for performing global metabolic profiling studies. In addition, a range of products used to collect these blood spots have also been studied for effects on the UPLC–MS background. Results: These preliminary data suggest that sample stability in this application is limited unless the cards are stored at, at least, -20°C and preferably at -80°C. In addition, the use of untreated cards is recommended to minimize incurred background interferences. Conclusion: Further investigations are necessary to improve the utility of this methodology in global metabolite profiling.