Bioanalysis Zone

A generic kit-based approach for quantifying monoclonal antibody drugs through direct digestion of discovery study samples

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In this application note, we have used the ProteinWorks™ eXpress Direct Digest Kit to simplify and streamline the workflow process using the same universal protocol and reagents for all monoclonal antibody drugs tested.

The ProteinWorks eXpress Direct Digest Kit was successfully used to purify and simultaneously quantify infliximab, adalimumab, bevacizumab, and trastuzumab from a typical set of standard curve and QC samples in rat plasma. The standardized, kit-based approach enables inexperienced users to immediately obtain meaningful data in discovery studies in order to make time sensitive and critical project decisions.

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