Analysis of oligonucleotides from biological samples is a challenging task. Due to the heterogeneous nature of the analytes, currently no generic bioanalytical approach is available to cover all the different therapeutically relevant oligonucleotide classes.
Bioanalysis is mainly conducted by LC/MS, hybridization-ELISA assay, qPCR, or AEX-HPLC with fluorescence detection. Depending of the analytical technique and the biological matrix, the extraction procedure is the most critical step. Here a comparison of the different methodologies used for the bioanalysis of oligonucleotides is shown, with a special focus on the properties of the PNA-HPLC assay that combines competitive sensitivity with an extraction free sample preparation procedure.
What will you learn?
- Analytical techniques suitable for bioanalysis of short oligonucleotide sequences
- Applications of the PNA-HPLC assay for oligonucleotide bioanalysis
Who may this interest?
- Scientists working in Pharma/Biotechs/Academia with oligonucleotide therapeutics
- Scientist in DMPK
- 1992-1997: Chemistry study at the University of Oldenburg (Germany)
- 2001-2003: Head of the Analytical Chemistry group at NOXXON Pharma AG, Berlin
- 1998-2000: Ph.D. work at the University of Oldenburg
- 2003-2007: Head of Analytical Chemistry at the Alnylam Pharmaceuticals with a focus on siRNA analytics and bioanalytics
- 2007-2011: Associate Director at the Roche Kulmbach RNA Center of Excellence. The focus of the analytical group was QC of the in house synthesis department, CMC development for clinical siRNA candidates, DMPK and tissue distribution analysis of siRNAs
- Since 2012: Director Analytics and Bioanalytics at the Axolabs GmbH in Kulmbach, Germany