Bioanalysis Zone

Increased confidence in protein quantitation: Quantitation of Infliximab in human serum with LC–MS/MS for clinical research


The therapeutic monoclonal antibody, Infliximab (IFX), is a widely used drug for the treatment of several inflammatory autoimmune diseases. However, approximately 10% of patients develop anti-infliximab antibodies (ATIs) rendering the treatment ineffective. Early detection of underexposure to unbound IFX would result in a timely switch of therapy, which could aid in the treatment of this disease.

In this webinar, a sensitive, robust and cost-effective method will be presented for the bioanalysis of IFX with LC–MS/MS by means of a target-based pre-analytical sample purification. Low volume and low cost of consumables/sample highlight its feasibility in preclinical studies.

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What will you learn?

  • How to develop a confident, robust, reliable, and sensitive quantitation assay for proteins
  • Ways to increase efficiency in sample preparation
  • The abiltiy to optimize LC and MS methods for performing protein quantitation assays with confidence
  • How to address critical challenges in a preclinical environment: from achieving sensitvity to reducing cost/sample

Who may this interest?

  • Bioanalytical Scientists
  • Sr. Scientists, Group Leaders, Directors, Sr. Directors
  • Large Pharma, small Pharma, Biopharma, CROs

For Research Use Only. Not for use in diagnostic procedures.


Mohsin El Amrani
Department of Clinical Pharmacy
Division of Laboratory Medicine and Pharmacy, University Medical Center Utrecht, Utrecht, The Netherlands

Mohsin El Amrani was born in Utrecht, the Netherlands on September 18, 1978. He obtained a high school degree at the ‘Delta Collage’ in Utrecht in 1995. In 1999 he completed a degree at ‘ROC Utrecht’, obtaining a diploma as a laboratory technician. In 2002 he obtained a Bachelor degree at the Institute of Life Sciences and Chemistry (Hogeschool van Utrecht) majoring in Analytical Chemistry. In 2014 he obtained a Master’s degree in Analytical Sciences at Vu University in Amsterdam. Currently he is working at UMCU where he holds a position as a research analyst and PhD candidate in proteomics.

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