Developing quantitation methods that can address critical challenges faced in quantitation of both biologics and small molecules require every bioanalytical scientist to achieve the most robust, reproducible, reliable and sensitive data for their target analytes in complex biological matrices. Liquid chromatography coupled to triple quadrupole (QQQ) mass spectrometers and high resolution accurate mass (HRAM) spectrometers are capable of delivering such sensitive targeted quantitation of both small molecules and biologics.
In this webinar, two LC–MS based quantitation workflows will be showcased for quantitation of insulin-like growth factors and other peptides. Both workflows highlight a robust sample preparation method for achieving optimal sensitivity. While one workflow highlights the robustness and high selectivity of a QQQ, the other will highlight HRAM MS offering the highest resolution and outstanding scan speed. Together, these biologics quanititation workflows enable every bioanalytical scientist and laboratory to achieve their scientific and business goals.
What will you learn?
- How to develop a confident, robust, reliable, and sensitive quantitation assay for proteins
- The parameters for choosing the right MS platform for the biologics quantitation workflow
- Optimization of LC and MS methods with high efficiency
- How to address critical challenges in establishing a biologics quantitation workflow: from achieving sensitivity and robustness to reducing cost/sample
Who may this interest?
- Bioanalytical Scientists
- Sr. Scientists, Group Leaders, Directors, Sr. Directors
- Large pharma, small pharma, biopharma, CROs
Application Demonstration Laboratory, Thermo Fisher Scientific
Rory Doyle works for Thermo Fisher (MA, USA) as the Demo Lab supervisor and has a BSc in Biochemistry from University College, Dublin (Ireland), MSc in Forensic Chemistry from University of Strathclyde (Glasgow, UK) and a MS in Clinical and Forensic Toxicology from University of Florida, Gainesville (FL, USA). He is certified as a Clinical Chemist with the National Registry of Clinical Chemists. He worked for Agilent Technologies (CA, USA) as the Clinical/Forensic Applications Manager, was the Associate Director of the LC–MS department at Quest Diagnostics (VA, USA) and was Deputy Chief Toxicologist at the Office of the Chief Medical Examiner in Washington (DC, USA). He specializes in small molecule and quantitative peptide and protein analysis, particularly in the clinical and forensic market.
Thermo Fisher Scientific
Kerry received her BSc degree in Chemistry from the University of Hartford (CY, USA) and her PhD in Analytical Chemistry from Purdue University (IN, USA) under the direction of Prof. Scott McLuckey. She was a postdoctoral fellow at Northeastern University (MA, USA) with Prof. John Engen where she studied HDX in combination with gas-phase ion/ion reactions. As an Applications Scientist at Thermo Fisher Scientific (MA, USA), Kerry developed novel LC–MS methods for clinically relevant analytes (therapeutic drugs, drugs of abuse and steroids). Kerry is currently a Product Specialist where she is responsible for the design and execution of novel experiments to demonstrate the ability of new chromatographic and mass spectrometric platforms to improve experimentation in laboratories across multiple markets and applications. She has published several articles in peer-reviewed journals for both small and large molecule anaylsis.
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