Over the years, LC–MS/MS has become the method of choice for detection in bioanalysis and many other areas. All of these application areas have stimulated the development of chromatographic separation material and hardware to improve the selectivity and sensitivity of LC–MS/MS in that last decade. However, in many instances, the chromatographic separation of isoforms and isobaric species can still be challenging, even when MS/MS is used for detection. SelexION® differential mobility separation technology (DMS) device can be used to separate analyte ions in the gas phase prior to MS analysis, and it can be used to enhance the selectivity of LC–MS analysis. Since DMS effectively separates ions in the gas phase, changing the properties of surrounding gas by controlled addition of vapors can lead to further increases in selectivity for analysis. Example of application areas where DMS was used to compliment the LC, or even eliminate the LC separation step, will be presented in this webinar.
Who may this interest?
- CROs, Pharma and Biotech companies focused on enhancing selectivity for LC–MS quantitative bioanalysis
- Mass spectrometrists and bioanalytical chemists
- Academicians and students interested in ion mobility techniques