In this application note, a two-dimensional chromatographic assay (2D LC or 2D UPLC) for mulitple reaction monitoring (MRM) quantification in protein bioanalysis is demonstrated.
During purification and in final formulation, proteins, including monoclonal antibodies and other biotherapeutic agents, are often in the presence of a low concentration of surfactant (<0.01%) to inhibit protein aggregation or adsorption.
The benefits of porous-shell columns are now available to scientists who are tasked to advance our knowledge about peptides, proteins and other biopolymers.
This poster presents two case studies were the SelexION™ Differential Mobility Separation Device was evaluated for the quantitation of therapeutic peptides.
Although the majority of compounds analyzed by HPLC have a mass below 1500 Da, there is a growing need to improve the performance of HPLC columns for the separation of peptides, proteins and protein drug conjugates.
This application note investigates the impact of column temperature on RPC of several globular proteins and monoclonal antibodies.
This application note summarizes performance characteristics of the quantitative ADA assay and described the validation performance of a Cut Point based, Screening, Titer and Confirmatory ADA assays.
Monitoring of the formation of Anti-drug antibody (ADA) to biological therapeutic is an integral component of pre-clinical and clinical trials.
Previously published methods of DNA quantification in tissue have employed homogenization/digestion of tissue followed by purification of DNA using silica-based minicolumns prior to assay.
In this case study, a fit-for purpose modification of the Total Ghrelin assay is described and analytical method qualification characteristics of the assay are presented.
