Definition of the week – Aqueous normal-phase LC

Posted In: Chromatography

  • Moderator
    fsgmanager on #3812

    Aqueous normal-phase LC: LC based on silica hydride-based stationary phases, where hydrophilic partitioning occurs via a gradient-elution program over a wide water-content range.

    Do you agree with this definition? Let us know below!

    Robert MacNeill on #7081

    I think it’s too condensed to be really deemed correct. The term is indeed synonymous with chromatography on silica hydride-based phases though – where there is typically the manifestation of both reversed-phase and normal phase interactions within a single gradient run, hence the ability to acceptably (retention, peak shape, selectivity etc) chromatograph compounds of widely varying polarity within said single run. I much prefer the definition given in the Bioanalysis Glossary:-
    A variant of normal phase liquid chromatography, i.e. the stationary phase is more polar than the mobile phase, but for which the mobile phase components and additives are water-miscible. HILIC and much chromatography on silica hydride-based phases are popular examples of ANP.

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