This application note from Waters discusses a robust and reliable IA-LC-HRMS method for the qualitative analysis and quantification of ado-trastuzumab emtansine (T-DM1, Kadcyla™).
Bioanalytical workflow for novel scaffold protein–drug conjugates: quantitation of total Centyrin protein, conjugated Centyrin and free payload for Centyrin–drug conjugate in plasma and tissue samples using liquid chromatography–tandem mass spectrometry
Alternative scaffold proteins have emerged as novel platforms for development of therapeutic applications. One such application is in protein–drug conjugates, which are analogous to antibody–drug conjugates. Find out more in this research article from Bioanalysis.
Read time: 3:45 mins
Find out more about the immunogenicity of antibody–drug conjugates (ADCs) in this column from Corinna Fiorotti, Business Development Director at BioAgilytix (NC, USA).
Immunoprecipitation middle-up LC–MS for in vivo drug-to-antibody ratio determination for antibody–drug conjugates
In this article from Bioanalysis, the authors present a middle-up LC–MS approach for DAR analysis using prelabeled capture beads and in-house fabricated slit-plates.
Development of Native and Denaturing LC-MS Methods for Characterization of Cysteine-Linked Antibody Drug Conjugates Using a Non-Toxic Mimic
Here we utilize a cysteine-linked antibody fluorophore conjugate to show how LC-MS methods may be optimized using a non-toxic ADC-mimic. The ADC-mimic was used to develop native and reduced SEC-MS conditions. The reduced mimic’s heavy and light chains, as well as its IdeS-proteolysis fragments, were used to develop reversed-phase LC-MS methods. A tryptic digest of the ADC mimic was used to develop a peptide mapping LC-MS method.
The global CRO, PPD, have been announced as the best CRO provider at the World ADC Awards for advancing cancer research.
Avoid preclinical testing failure by understanding the complex bioanalytical and toxicology challenges associated with ADC drug development and unique ways to overcome them.
In this article, the authors aim to provide an insight to the outsource-ability of high-resolution MS-based DMPK assays.
Antibody–drug conjugate bioanalysis using LB-LC–MS/MS hybrid assays: strategies, methodology and correlation to ligand-binding assays
In this article, the authors aim to provide technical guidance for scientists conducting various hybrid assays for ADC bioanalysis.