Browsing: ELISA

Large molecule quantification by LC–MS PPD IMage

Monoclonal antibody (mAb) based biotherapeutics have emerged as a dominant class of approved medicines during the past decade in both market share and variety of target diseases. Ligand binding assays (LBAs), such as enzyme-linked immunosorbent assays (ELISA), have been the gold standard for the bioanalysis of mAbs in support of non-clinical and clinical trials. While conventional LBAs provide highly sensitive and specific detection for the quantitation of mAbs, combining an LBA with liquid chromatography tandem mass spectrometry (LC-MS/MS) detection offers additional orthogonal dimensions of selectivity/specificity, extending the assay utility to a multiplexed format otherwise not achievable by LBA alone.

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In this informative infographic, we summarize the top 5 techniques for studying immuno-oncology including: flow cytometry, enzyme-linked immunospot, ELISA, electrochemiluminescence by meso scale discover and single moleucle array.

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