In this informative infographic, we summarize the top 5 techniques for studying immuno-oncology including: flow cytometry, enzyme-linked immunospot, ELISA, electrochemiluminescence by meso scale discover and single moleucle array.
The facts, frustrations and future hopes for immuno-oncology: a podcast with Dr Dominic Warrino (KCAS)
As part of our focus, we present this podcast where Dr Dominic Warrino discusses how immuno-oncology fits into the bioanalytical field and the technologies involved.
Researchers from King Saud University (Riyadh, Saudi Arabia) have developed a new ELISA technique, with increased sensitivity and selectivity, for the simple monitoring of bevacizumab levels in blood plasma.
Development and validation of an impedance biosensor for point-of-care detection of vascular cell adhesion molecule-1 toward lupus diagnostics
Learn more about the potential future application of point-of-care biosensors in clinical and at-home monitoring of autoimmune diseases, such as lupus in this article from Future Science OA
Development and validation of an electrochemiluminescent ELISA for quantitation of oral insulin tregopil in diabetes mellitus serum
This methodology discusses how a sensitive ELISA method could be used to support accurate measurement of Tregopil insulin and its metabolites.
Researchers have identified a panel of biomarkers in serum samples that could accurately detect the early stages of pancreatic cancer.
3-(4-Hydroxyphenyl)propionic acid: the forgotten detection substrate for ligand-binding assay-based bioanalysis
In this review article the authors outline the increase in sensitivity and dynamic range when using fluorogenic 3-(4-hydroxyphenyl)propionic acid in preference of other conventional colorimetric ELISA, LBA technologies and LC-MS assays.
Validation of a biotherapeutic immunoaffinity-LC–MS/MS assay in monkey serum: ‘plug-and-play’ across seven molecules
In this research article the authors describe the validation of an immunoaffinity (IA)-LC–MS/MS method to quantify a monoclonal antibody biotherapeutic in cynomolgus monkey serum. The results from this validation study demonstrate the ‘plug-and-play’ nature of the IA-LC–MS/MS mAb framework peptide assay format.
Highly specific and sensitive immunoassay for the measurement of prostaglandin E2 in biological fluids
In this article a team from Peking Union Medical College Hospital and Chengdu University of Traditional Chinese Medicine present a method for producing highly specific anti-PGE2 antibodies, a key challenge in the development of specific immunoassay. Furthermore the team establishes a highly sensitive ELISA, which could be applied to directly analyze PGE2 at pg/ml levels.
This special report provides an overview of ligand-binding assays, from their origin and evolution to commonly used formats.