Researchers have developed a paper chip that utilizes ionic antibody probes to diagnose disease – the device could be used at home to test for malaria and other diseases.
New analytical application of antibody-based biosensor in estimation of thyroid-stimulating hormone in serum
In this study, an immunosensor-based, kinetic-exclusion analysis (KinExA) was used for thyroid-stimulating hormone estimation.
Sensitive assays enable detection of serum IgG antibodies against Clostridium difficile toxin A and toxin B in healthy subjects and patients with Clostridium difficile infection
The authors invesitgate titer-based assays as a means of assessment of antitoxin A and antitoxin B IgG antibodies as potential biomarkers to identify patients with CDI at increased risk for CDI recurrence.
In this commentary Jeffrey Duggan (Boehringer–Ingelheim) reflects on the special considerations for method development and validation of proteolytic digestion LC-MS immunocapture methods, detailing the procedures used at Boehringer–Ingelheim to generate additional data to establish the reliability of their assays when applied to the varying matrices and possible interferents encountered in their clinical studies.
Highly specific and sensitive immunoassay for the measurement of prostaglandin E2 in biological fluids
In this article a team from Peking Union Medical College Hospital and Chengdu University of Traditional Chinese Medicine present a method for producing highly specific anti-PGE2 antibodies, a key challenge in the development of specific immunoassay. Furthermore the team establishes a highly sensitive ELISA, which could be applied to directly analyze PGE2 at pg/ml levels.
A clinical biomarker assay to quantitate thymic stromal lymphopoietin in human plasma at sub-pg/ml level
This research article details the development and validation of a ultrasensitive electrochemiluminescence assay for the measurement of thymic stromal lymphopoietin.
Crystal City VI: Assay Criteria and Level of Validation Required for Biomarkers Will Depend Upon the Intended Purpose of the Assay
In this article Jennifer Zimmer (Alturas Analytics Inc) details the recent AAPS Crystal City VI meeting during which the draft bioanaytical method validation guidance and how it relates to biomarker assays was discussed.
Cameron Low interviews Rob Durham, Director of Field Applications Scientists, North America, Gyros on automation of assays and the Gyros Immunoassay.
Recommendations for adaptation and validation of commercial kits for biomarker quantification in drug development
In this white paper recommendations for the use of immunoassay kits are provided. These recommendations aim to standardize and harmonize user practices, contributing to reliable biomarker data from commercial immunoassays, thus, enabling properly informed decisions during drug development.
Accelerating clinical flow cytometry studies using qualified, modular and transferrable immunophenotyping assays
Part 1 of webinar series
Reducing the development time and cost of immunophenotyping studies through the application of pre-assembled core immunophenotyping panels.