Browsing: immunoassay

Large molecule analysis by LC–MS
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In this commentary Jeffrey Duggan (Boehringer–Ingelheim) reflects on the special considerations for method development and validation of proteolytic digestion LC-MS immunocapture methods, detailing the procedures used at Boehringer–Ingelheim to generate additional data to establish the reliability of their assays when applied to the varying matrices and possible interferents encountered in their clinical studies.

Features Chinese new year image 2
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In this article a team from Peking Union Medical College Hospital and Chengdu University of Traditional Chinese Medicine present a method for producing highly specific anti-PGE2 antibodies, a key challenge in the development of specific immunoassay. Furthermore the team establishes a highly sensitive ELISA, which could be applied to directly analyze PGE2 at pg/ml levels.

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