We talk to Vellalore Kakkanaiah about his work using flow cytometry in adoptive cell therapy in this interview as part of our author’s perspective series.
In this Bioanalysis Zone interview, Pegah Jalili (MilliporeSigma) discusses her current work in analytical R&D of biotherapeutic characterization and quantification, and what she thinks the future looks like for this field of bioanalysis.
An author’s perspective: Zhengqi Ye on approaches to measure protein binding of enzymatically unstable compounds
We talk to Zhengqi Ye about the approaches she uses to measure protein binding of enzymatically unstable compounds in plasma in this interview as part of our author’s perspective series.
An author’s perspective: Wenying Jian on bioanalytical workflow for novel scaffold protein–drug conjugates
We talk to Wenying Jian about her work on the quantitation of total Centyrin protein using liquid chromatography–tandem mass spectrometry in this interview as part of our author’s perspective series.
Quantification of phenolic acid metabolites in humans by LC–MS: a structural and targeted metabolomics approach
In this research article from Bioanalysis, the authors quantified and identifed over 50 analytes from human biological matrices by HPLC, NMR and LC–MS to obtain structural information on phenolic acid metabolites.
Bioanalytical workflow for novel scaffold protein–drug conjugates: quantitation of total Centyrin protein, conjugated Centyrin and free payload for Centyrin–drug conjugate in plasma and tissue samples using liquid chromatography–tandem mass spectrometry
Alternative scaffold proteins have emerged as novel platforms for development of therapeutic applications. One such application is in protein–drug conjugates, which are analogous to antibody–drug conjugates. Find out more in this research article from Bioanalysis.
From the quantification and validation of biomarkers to the integration of novel technology in bioanalytical workflows, as the year comes to a close we’re looking back on the bioanalytical highlights from 2018.
Webinar available to view on demand. The application of differential mobility spectrometry to overcome selectivity and sensitivity challenges for both small and large molecule quantitation in bioanalysis.
Available to view on demand. We present a rare opportunity to see experts from across the pharmaceutical and CRO industries sharing perspectives on new technology integration in the field of bioanalysis.
MetaboQuan-r for bile acids in human serum: a rapid, targeted UPLC-MS/MS method for metabolomic research studies
Bile acids are an important class of biological molecules that are generated in the liver and play a central role in various biological functions, including cholesterol homeostasis. Quantitative analysis for research of bile acids using LC-MS is complicated by the presence of many isomeric compounds.Historically, this has meant that throughput has been compromised in order to separate these isomeric compounds. Here we demonstrate how these compounds can be separated, without compromising throughput, using Waters® CORTECS UPLC Technology combined with negative mode electrospray ionization mass spectrometry. This application note is also part of a MetaboQuan-R method package.