Browsing: LC–MS/MS

Large molecule quantification by LC–MS PRA

Encenicline (EVP-6124), shown in Figure 1, is a potent orthosteric agonist of the α7 nicotinic acetylcholine receptor (α7 nAChR). This compound is being developed with the potential clinical indication
to treat cognitive impairment in patients with schizophrenia or Alzheimer’s disease (AD).

The primary circulating non-conjugated metabolites of encenicline are encenicline N-oxide, formed by oxidation of the quinuclidine nitrogen atom, and encenicline acid metabolite, a product formed through hydrolysis of an amide bond. Metabolite profiling has also indicated the presence of an N-glucuronide as well as other metabolites.

Sample preparation 1EBF 2017 BioSPME Matrix Effect Poster-1

Biocompatible solid phase micro extraction (BioSPME) is a sample preparation method that allows for the extraction of various analytes of interest from complex biological matrices without significant co-extraction of common interferences including salts, proteins, and phospholipids. Analytes can be recovered by simply desorbing within an organic solvent prior to analysis. Significant reduction in matrix interferences allows the user to focus on the separation of analytes during method development.

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