Browsing: LGC

Immunogenicity BZ - LGC A collaborative relationship between the Analytical Scientist and the Statistician.pptx - AutoRecovered

With vast amounts of data being generated it is important to make the right decision when it comes to appropriate cut-points. In this presentation, Alexandra Hawes (Principal Scientist, LGC, UK), outlines the collaborative process in the context of updated recommendations for statistical analyses. She also discusses the importance of the analytical scientists understanding of how the statistical analysis should be applied and its appropriateness for analytical data. Also included in the presentation are three case studies that illustrate the importance of the collaborative relationship between statisticians and scientists.

Large molecule quantification by LC–MS LGC_Glu-c_an_orthogonal_and_alternative_enzyme_for_protein_quantitation_SS

To date, almost all protein LC-MS/MS based bioanalysis has involved the use of trypsin to cleave biopharmaceuticals into manageable peptides for quantitation. In some cases, where trypsin does not generate useful peptide sequences, a different cleavage agent is required. Glu-C is an enzyme that is frequently used for protein characterisation, and cuts proteins on the C-terminal side of the aspartic and glutamic acid residues. These two molecules are acidic amino acids, whereas the ubiquitous trypsin cleaves at the basic residues (lysine and arginine), therefore making this an orthogonal cleavage enzyme.

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