Browsing: ligand-binding assay

LBA LBA infographic

As part of our Spotlight on LBA, we carried out a survey to gain an insight into the trends, challenges and developments associated with this technique. We would like to thank everyone who was able to share their thoughts by taking part in the survey – you can see the results below in our infographic.

Large molecule quantification by LC–MS

In this application note, Yun Alelyunas (Waters Corporation) describes an LC-HRMS method for high sensitivity bioanalysis of monoclonal antibodies by direct mass measurement at the intact level for whole molecule quantification. This emerging approach can serve as an alternative or complement to traditional methods such as ligand binding assay for protein quantification. Methodology and guidance for evaluating and optimizing data processing parameters are discussed in detail.