In this Learn with Lin article, Zone Leader Lin Wang (Covance, NJ, USA) explores some of the key terms in LC–MS that form the foundations of a good understanding of the bioanalytical applications of this technique.
Browsing: matrix effects
In this perspective piece, a general discussion on the importance of these matrix effects is provided as well as how to properly address them in the method-development and validation stages of regulated bioanalysis.
Issue 23 of Bioanalysis volume 9 is now available, including an editorial discussing the matrix effects, a methodology on assay signaling and a White Paper on the rise of hybrid LBA/LCMS immunogenicity assays.
LC–MS quantification of protein drugs: validating protein LC–MS methods with predigestion immunocapture
The purpose of this paper is to describe validation experiments that measure immunocapture efficiency, digestion efficiency, matrix effect and selectivity/specificity that can be used during method optimization and validation to test the resistance of the method to these potential interferences. The designs and benefits of these experiments are discussed in this report using an actual assay case study.
A simple, effective approach for rapid development of high-throughput and reliable LC–MS/MS bioanalytical assays
In this article, the authors describe a simple, effective approach to achieve accelerated method development of high-throughput and reliable LC–MS/MS assays for regulated bioanalysis.
Finding the needle in the haystack: A simple path to matrix removal and robust solid-phase extraction methods
In this seminar, we will outline several different approaches to sample clean-up using a variety of SPE tools.
This article highlights the impact that sample matrix effects can have on LC/MS response and discusses two novel approaches to reduce it.
In this article – the third installment on our IMS feature from the Mass Spectrometry Research Center at Vanderbilt University – a matrix toolbox for MALDI IMS is presented.
In this commentary Jeffrey Duggan (Boehringer–Ingelheim) reflects on the special considerations for method development and validation of proteolytic digestion LC-MS immunocapture methods, detailing the procedures used at Boehringer–Ingelheim to generate additional data to establish the reliability of their assays when applied to the varying matrices and possible interferents encountered in their clinical studies.
An investigation of the comparability of commercially sourced plasma and pharmaceutical study plasma, using total protein concentration
In this research article the authors evaluate if there is a significant difference between commercially supplied plasma and study prepared plasma, and hence whether control plasma from commercial suppliers is routinely suitable for use in preparing study equivalent validation samples in bioanalysis, proteomics and biomarker analysis.