Browsing: matrix effects

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Issue 23 of Bioanalysis volume 9 is now available, including an editorial discussing the matrix effects, a methodology on assay signaling and a White Paper on the rise of hybrid LBA/LCMS immunogenicity assays.

Regulated bioanalysis

The purpose of this paper is to describe validation experiments that measure immunocapture efficiency, digestion efficiency, matrix effect and selectivity/specificity that can be used during method optimization and validation to test the resistance of the method to these potential interferences. The designs and benefits of these experiments are discussed in this report using an actual assay case study.

Large molecule analysis by LC–MS

In this commentary Jeffrey Duggan (Boehringer–Ingelheim) reflects on the special considerations for method development and validation of proteolytic digestion LC-MS immunocapture methods, detailing the procedures used at Boehringer–Ingelheim to generate additional data to establish the reliability of their assays when applied to the varying matrices and possible interferents encountered in their clinical studies.

Drug Discovery and Development

In this research article the authors evaluate if there is a significant difference between commercially supplied plasma and study prepared plasma, and hence whether control plasma from commercial suppliers is routinely suitable for use in preparing study equivalent validation samples in bioanalysis, proteomics and biomarker analysis.

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