Browsing: molecule

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Bile acids are an important class of biological molecules that are generated in the liver and play a central role in various biological functions, including cholesterol homeostasis. Quantitative analysis for research of bile acids using LC-MS is complicated by the presence of many isomeric compounds.Historically, this has meant that throughput has been compromised in order to separate these isomeric compounds. Here we demonstrate how these compounds can be separated, without compromising throughput, using Waters® CORTECS UPLC Technology combined with negative mode electrospray ionization mass spectrometry. This application note is also part of a MetaboQuan-R method package.

Large molecule quantification by LC–MS

In this application note, Yun Alelyunas (Waters Corporation) describes an LC-HRMS method for high sensitivity bioanalysis of monoclonal antibodies by direct mass measurement at the intact level for whole molecule quantification. This emerging approach can serve as an alternative or complement to traditional methods such as ligand binding assay for protein quantification. Methodology and guidance for evaluating and optimizing data processing parameters are discussed in detail.