We are revisiting this area to explore how the oligonucleotide field has actually developed and provide a critical review of where we are now.
MetaboQuan-r for bile acids in human serum: a rapid, targeted UPLC-MS/MS method for metabolomic research studies
Bile acids are an important class of biological molecules that are generated in the liver and play a central role in various biological functions, including cholesterol homeostasis. Quantitative analysis for research of bile acids using LC-MS is complicated by the presence of many isomeric compounds.Historically, this has meant that throughput has been compromised in order to separate these isomeric compounds. Here we demonstrate how these compounds can be separated, without compromising throughput, using Waters® CORTECS UPLC Technology combined with negative mode electrospray ionization mass spectrometry. This application note is also part of a MetaboQuan-R method package.
In this application note, Yun Alelyunas (Waters Corporation) describes an LC-HRMS method for high sensitivity bioanalysis of monoclonal antibodies by direct mass measurement at the intact level for whole molecule quantification. This emerging approach can serve as an alternative or complement to traditional methods such as ligand binding assay for protein quantification. Methodology and guidance for evaluating and optimizing data processing parameters are discussed in detail.
2D-LC–MS/MS to measure cleaved high-molecular-weight kininogen in human plasma as a biomarker for C1-INH-HAE
This research article describes a reagent-free LC–MS assay that has been developed for the quantitation of cHMWK, which was shown to be elevated in plasma of C1-INH-HAE patients due to C1-INH deficiency relative to that of healthy subjects.
Microfluidics for macromolecules: leveraging microflow LC/MS for quantitation of therapeutic peptides
Available to view on demand
A variety of applications focusing on the quantitation of therapeutic (xenobiotic) peptides using the Waters ionKey mass spectrometer will be presented.