In this review from Bioanalysis, the authors concisely introduce coated blade spray’s operational fundamentals and to consider how it correlates/contrasts with existing direct-to-MS technologies suitable for bioanalytical applications.
An author’s perspective: Germán Augusto Gómez-Ríos on shifting the paradigm of direct sample introduction to MS
We talk to Germán Augusto Gómez-Ríos about shifting the paradigm of direct sample introduction to MS.
Evaluation of Recombinant, Chemically Treated Trypsin in Proteomics and Protein Characterization Assays
We compared recombinant trypsin to native-source in various MS workflows.
Native source trypsin is dimethylated to prevent autolysis and TPCK treated to inhibit chymotrypsin.
Native and recombinant trypsins gave similar amounts of proteins and peptides identified in a complex mixture.
TIC of a single-protein digest revealed more complete peptide processing by recombinant trypsins relative to native trypsin.
Trypsin dimethylation leads to slower processing but with fewer autolytic peptides present.
Learn about the fundamentals of ion mobility mass spectrometry (IMS) and the benefits of collisional-cross…
Chromatography and electrospray ionization mass spectrometry utilized to determine specific lipid biomarkers for breast cancer.
UHPLC-MS/MS bioanalysis of urinary DHEA, cortisone and their hydroxylated metabolites as potential biomarkers for CYP3A-mediated drug–drug interactions
In this research article the authors develop a UHPLC-MS/MS assay to quantify urinary dehydroepiandrosterone (DHEA), 7β-hydroxy-DHEA, cortisone and 6β-hydroxycortisone as potential biomarkers to predict CYP3A activity.
Analysis of mass spectrometry data leads researchers to potentially identify new antibiotics compounds
A team of computer scientists have developed a new method identifying new antibiotic compounds from previously unexplored mass spectrometry data.
New diagnostic test for cocaine developed by researchers from the University of Surrey’s Department of Chemistry (UK).
Researchers from Cardiff University (Cardiff, UK) have developed a non-invasive method that could be utilized to predict the risk of an individual presenting a severe form of liver disease.
Challenges for the in vivo quantification of brain neuropeptides using microdialysis sampling and LC–MS
In this paper, the authors discuss the challenges of quantifiying brain neuropeptides using microdialysis sampling and LC–MS and some possible approaches to minimize their interferences.