Evaluation of Recombinant, Chemically Treated Trypsin in Proteomics and Protein Characterization Assays
We compared recombinant trypsin to native-source in various MS workflows.
Native source trypsin is dimethylated to prevent autolysis and TPCK treated to inhibit chymotrypsin.
Native and recombinant trypsins gave similar amounts of proteins and peptides identified in a complex mixture.
TIC of a single-protein digest revealed more complete peptide processing by recombinant trypsins relative to native trypsin.
Trypsin dimethylation leads to slower processing but with fewer autolytic peptides present.