In this series of short, recorded presentations a team from Waters (MA, USA) discuss fundamental and practical details regarding large molecule quantification.
Flow cytometric immunophenotyping of PBMCs and quantification of cytokine production from patients in a peptide vaccine study
KCAS, LLC optimized a primary and secondary stimulation assay of Peripheral Blood Mononuclear Cell (PBMC)
samples from a clinical trial in order to characterize the immune response to a multi-valent peptide vaccine. In partnership with our pharmaceutical sponsor, an agreed upon set of samples were tested by KCAS, LLC to optimize a culture method, culture time course, stimulation, and re-stimulation. These samples were also used to optimize surface and intracellular staining of the following panel: CD45, CD3 CD8, PD1, CCR7, IFNγ, TNFα, IL-2 and viability dye. Upon review of the initial optimized method, the full cohort of patient samples were cultured, treated, stained, and analyzed.
A sensitive method for the quantitation of the peptide-based glucagon-like peptide-1 receptor agonist liraglutide in plasma using microfluidics chromatography tandem MS
In this article, the authors discuss an LC–MS/MS assay for the quantitation of liraglutide, a peptide-based injectable glucagon-like peptide-1 receptor agonist, as a convenient alternative to the enzyme-linked immunosorbent assay.
Waters’ team of bioanalysis experts developed the Peptide & Protein Bioanalysis Boot Camp – a…
Developing novel methods for protein, peptide and metabolite analysis: an interview with Rainer Bischoff
Watch time: 6:47 mins
Rainer Bischoff (University of Groningen; Groningen, The Netherlands) explains his current research focus with an emphasis on developing novel methods for protein, peptide and metabolite analysis in this video interview.