An isobaric labeling technique has been developed to amplify signals for comprehensive quantitative phosphoproteomic characterization of small cells populations.
2018 White Paper on Recent Issues in Bioanalysis: ‘A global bioanalytical community perspective on last decade of incurred samples reanalysis (ISR)’ (Part 1 – small molecule regulated bioanalysis, small molecule biomarkers, peptides & oligonucleotide bioanalysis)
This part of the 2018 White Paper published in Bioanalysis discusses the recommendations for LC–MS for small molecules, peptides, oligonucleotides and small molecule biomarkers.
An author’s perspective: Amanda King-Ahmad on quantitation of the peptide-1 receptor agonist liraglutide
We talk to Amanda King-Ahmad about her work on the peptide-based glucagon-like peptide-1 receptor agonist liraglutide in this interview as part of our author’s perspective series.
To date, almost all protein LC-MS/MS based bioanalysis has involved the use of trypsin to cleave biopharmaceuticals into manageable peptides for quantitation. In some cases, where trypsin does not generate useful peptide sequences, a different cleavage agent is required. Glu-C is an enzyme that is frequently used for protein characterisation, and cuts proteins on the C-terminal side of the aspartic and glutamic acid residues. These two molecules are acidic amino acids, whereas the ubiquitous trypsin cleaves at the basic residues (lysine and arginine), therefore making this an orthogonal cleavage enzyme.
Waters’ team of bioanalysis experts developed the Peptide & Protein Bioanalysis Boot Camp – a…
A team from the University of Toronto (ON, Canada) has developed a method to produce geometric isoforms of drugs, potentially leading to improved efficiency.
Researchers have identified the DAG peptide as a potential biomarker for Alzheimer’s disease development.
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Capillary microsampling in nonclinical safety assessment: practical sampling and bioanalysis from a CRO perspective
In this special report, the authors discuss the initial considerations before using capillary microsampling, study design and some practicalities of sample collection.
Challenges for the in vivo quantification of brain neuropeptides using microdialysis sampling and LC–MS
In this paper, the authors discuss the challenges of quantifiying brain neuropeptides using microdialysis sampling and LC–MS and some possible approaches to minimize their interferences.