Learn more about the positive impact of upfront systematic screening and characterization of conjugated critical reagent attributes on assay robustness in this methodology article in Bioanalysis.
In this series of short, recorded presentations a team from Waters (MA, USA) discuss fundamental and practical details regarding large molecule quantification.
Microflow UPLC and high-resolution MS as a sensitive and robust platform for quantitation of intact peptide hormones
In this research article the authors discuss the use of high-resolution mass spectrometry for basic characterization and reliable quantitation in complex matrices.
A new proteomics technique, termed ubiquitin clipping, has been developed to further understanding of changes that control protein function and their role in healthy and disease states.
An author’s perspective: Zhengqi Ye on approaches to measure protein binding of enzymatically unstable compounds
We talk to Zhengqi Ye about the approaches she uses to measure protein binding of enzymatically unstable compounds in plasma in this interview as part of our author’s perspective series.
Current methods for the treatment of cancer include radiation, chemotherapy and surgical intervention, but alternative treatment modalities exist should these traditional methods prove unsuccessful. In this blog post, Dominic Warrino (KCAS; KS, USA), shares his insights into immuno-oncology, and discusses two case studies utilizing antibody drug conjugates and cellular therapies.
Opportunities and challenges for hybrid immunoaffinity LC–MS approach for quantitative analysis of protein biomarkers
Learn more about opportunities and challenges for hybrid immunoaffinity LC–MS approach for quantitative analysis of protein biomarkers in this article from Future Science OA.
Introduction Low sensitivity methods in protein LC–MS bioanalysis commonly utilise a ‘bottom-up’ approach. However, lack…
In this application note, Yun Alelyunas (Waters Corporation) describes an LC-HRMS method for high sensitivity bioanalysis of monoclonal antibodies by direct mass measurement at the intact level for whole molecule quantification. This emerging approach can serve as an alternative or complement to traditional methods such as ligand binding assay for protein quantification. Methodology and guidance for evaluating and optimizing data processing parameters are discussed in detail.