A research team from Brazil has combined two techniques to identify over 10, 000 proteins expressed in oligodendrocytes in an effort to better understand the causes of schizophrenia.
Evaluation of Recombinant, Chemically Treated Trypsin in Proteomics and Protein Characterization Assays
We compared recombinant trypsin to native-source in various MS workflows.
Native source trypsin is dimethylated to prevent autolysis and TPCK treated to inhibit chymotrypsin.
Native and recombinant trypsins gave similar amounts of proteins and peptides identified in a complex mixture.
TIC of a single-protein digest revealed more complete peptide processing by recombinant trypsins relative to native trypsin.
Trypsin dimethylation leads to slower processing but with fewer autolytic peptides present.
Webinar Q and A follow up: Practical considerations in the design of quantitative targeted methods in proteomics
Follow-up of the live webinar Q and A session
In this mini-webinar you will learn about different LC-MS/MS approaches for quantitative protein bioanalysis with focus on biopharmaceuticals, biomarkers and targeted proteomics.
Attendees will learn our laboratory’s approach for designing, calibrating, and implementing protein quantitation assays in proteomics.
Metabolomic profiling could allow identification of cancer subtypes and precision therapy.
Analysis of mass spectrometry data leads researchers to potentially identify new antibiotics compounds
A team of computer scientists have developed a new method identifying new antibiotic compounds from previously unexplored mass spectrometry data.
Collaborative research from Kuramoto University, Tohoku University and the National Cancer Center of Japan (all Japan) has identified biomarkers found in blood that could improve the detection of pancreatic cancer.
Advancing SRM-based proteomics for detection and quantitation of histone modifications in scarce clinical specimens
In this webinar, we will discuss ongoing technology improvements for targeted histone analysis in clinical samples.
A study in Russia has devised a method to diagnose respiratory conditions in newborn babies by identifying proteomes in urine samples.