Browsing: selectivity

Regulated bioanalysis

The purpose of this paper is to describe validation experiments that measure immunocapture efficiency, digestion efficiency, matrix effect and selectivity/specificity that can be used during method optimization and validation to test the resistance of the method to these potential interferences. The designs and benefits of these experiments are discussed in this report using an actual assay case study.

Large molecule analysis by LC–MS

In this commentary Jeffrey Duggan (Boehringer–Ingelheim) reflects on the special considerations for method development and validation of proteolytic digestion LC-MS immunocapture methods, detailing the procedures used at Boehringer–Ingelheim to generate additional data to establish the reliability of their assays when applied to the varying matrices and possible interferents encountered in their clinical studies.

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