We talk to Laura Rowe about her work using UHPLC to analyse protein glycosylation in this interview as part of our author’s perspective series.
This review article from Bioanalysis> introduces the differences between UHPLC and HPLC, and reviews the use of UHPLC in the last 5 years when studying the glycosylation of proteins and glycoprofiling biopharmaceuticals.
Validated UHPLC–MS/MS method for quantification of doxycycline in abdominal aortic aneurysm patients
Read more about this development and validation of a selective, specific, simple and rapid UHPLC–MS/MS method for doxycycline, in our featured article from Bioanalysis.
Development of a UHPLC–MS/MS (SRM) method for the quantitation of endogenous glucagon and dosed GLP-1 from human plasma
In this research article, the authors discuss a LC–MS/MS method that offers a viable alternative to immunoassays for the quantitation of endogenous glucagon, dosed glucagon and/or dosed GLP-1.
UHPLC-MS/MS bioanalysis of urinary DHEA, cortisone and their hydroxylated metabolites as potential biomarkers for CYP3A-mediated drug–drug interactions
In this research article the authors develop a UHPLC-MS/MS assay to quantify urinary dehydroepiandrosterone (DHEA), 7β-hydroxy-DHEA, cortisone and 6β-hydroxycortisone as potential biomarkers to predict CYP3A activity.
A simple, effective approach for rapid development of high-throughput and reliable LC–MS/MS bioanalytical assays
In this article, the authors describe a simple, effective approach to achieve accelerated method development of high-throughput and reliable LC–MS/MS assays for regulated bioanalysis.
In this article, the authors discuss how some novel chromatographic approaches will be considered in terms of what they can offer the bioanalysts.
Researchers have developed a sensitive urine test for cannabinoids, which detects substances at low levels – akin to those that would be found in second-hand marijuana exposure.
DBS sampling in imatinib therapeutic drug monitoring: from method development to clinical application
In this research article a DBS sampling method for the measurement of Imatinib plasma concentrations using UHPLC–MS/MS was developed and validated.
Interview with Denise Wallworth (Sigma-Aldrich) on chiral separation, chromatography and potential future changes in LC-MS workflows
Cameron Low interviews Denise Wallworth, Tactical Marketing Manager, Sigma-Aldrich, on what she believes the main challenges are when developing chiral methods, whether the use of alternate techniques to U/HPLC will increase and more.