Antibody-free PRISM SRM for multiplexed protein quantification: is this the new competition for immunoassays in bioanalysis?

“PRISM–SRM affords a unique protein quantification technology for biomarker verification and systems biologystudies where a large number of proteins need to be quantified from a set number of samples.” In this postgenomic era there is a great demand in biomedical research for highly sensitive technologies that enable multiplexed detection and quantification of hundreds of low-abundance proteins and their modifications. For example, discovering effective biomarkers for improved prognosis/diagnosis of human diseases requires such technologies to rapidly verify and validate protein candidates. Traditionally, sensitive detection of low-abundance proteins (e.g., pg/ml to low ng/ml levels of proteins in human plasma/serum) has primarily relied...