Biomarker bioanalysis: road to the future
Innovative quantitation and assay qualification strategy are presented. Interference, sample collection procedure, Interference, sample collection procedure, intra- and inter-subject variability are discussed for LC-MS/MS for quantitation of fatty acid amide, biomarkers for fatty acid amide hydrolase. Stability and sample collection conditions and difficult to separate isomers are discussed for the LC-MS/MS analysis of Leukotriene B4 in human sputum. Relative quantitation was used for HRMS of apolipoprotein C3. Assay sensitivity and interference from other isobaric plasma oxysterols LC-MS/MS Quantification of 4β-hydroxycholesterol and cholesterol.
What will you learn?
- Bioanaytical quantitation of small and large molecules biomarkers
- HRMS
- Relative quantitation
- Derivatization for sensitivity enhancement
- Tiered approach.
Who should attend?
- Bioanalytical Scientists
- Laboratory Managers/Group Leaders/Senior Scientists
- Bioanalytical Laboratory Directors.
Speaker:
Naidong Weng
Scientific Director Head of US East Coast Bioanalytical and Pharmacokinetics
Janssen Fellow
Dr Weng has 97 peer-reviewed journal publications, 10 book chapters, over 100 posters and over 25 podium presentations. He also co-edited one book on bioanalysis (Eliminating bottlenecks for efficient bioanalysis: practices and applications in drug discovery and development, 2014) and one Special Focus Issue of Bioanalysis on Bioanalytical Laboratory Structure and Management (2014). He has been teaching short courses on DMPK at ASMS since 2011. He serves on the editorial advisory boards for two international scientific journals as well as on various scientific/organizational committees, most recently as the chairman for CPSA Shanghai 2013.
