Bioanalysis Zone

How can we improve ion-exchange separations in LC?

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“…by decreasing the particle size and increasing the pressure rating an increase of a factor of 2 in efficiency can be achieved, without sacrificing analysis time.”

Ion-exchange chromatography (IEX) emerged as an analytical technique for the separation of ions and ionizable analytes in 1975, when Small et al. described a novel approach to suppress counter ions in the mobile phase prior to conductivity detection [1]. Nowadays, dedicated instrumentation is available and compatible with normal bore (4.6 mm i.d.) and capillary (400 µm i.d.) column formats. Whereas the technology was initially applied only for the analysis of small inorganic anions and cations, its scope has expanded and IC is now commonly applied to analyze a wide range of species, including organic acids and aliphatic amines, and a wide range of biomolecules including antibodies, intact proteins, peptides and carbohydrates, among others.

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