Hans Stieltjes (Janssen Research & Development) has kindly provided Bioanalysis Zone a preview of his team’s novel research on non-capillary microsampling of plasma in rat and mice in support of GLP studies. Please note this work is pending peer-review.
Background: Different liquid microsampling techniques to obtain plasma samples have created great interest from toxicokinetic and pharmacokinetic scientists [1, 2]. These techniques enable reduction of sample volumes for exposure assessment in rodents and, consequently, serial profiles can be obtained from a single animal. In GLP safety studies, microsampling can remove the use of satellite animals. Although the full potential to reduce the sample volume is obtained with blood sampling and analysis, plasma remains the preferred matrix for exposure analysis. The current industry trend is to collect a small blood sample in capillaries. At Janssen, however, we initiated the collection of small volumes of blood (50—60 µL) into an appropriate adapted recipient to generate an accurate volume of 10 µL plasma in a more classical way.