A novel quantitative method for recovering precipitated uric acid in urine and analysis by LC–MS/MS


Abstract Aim: Precipitation of uric acid (UA) in human urine had caused poor recovery and underestimation of UA in study samples. Results: A UA recovery method was developed for analyzing frozen urine samples that potentially contain precipitated UA. Samples were treated with 3% v/v of a solution containing 8 M potassium hydroxide and 20% v/v of ammonium thioglycolate. The high pH enables rapid dissolution of UA crystals, while the thioglycolate protects UA from oxidation at high pH. The treated samples were diluted 20-fold with 50 mM ammonium chloride and analyzed immediately by LC–MS/MS or stored at -20°C prior to analysis....

To view this content, please register now for access

It's completely free