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Validation of methods for determining pediatric midazolam using wet whole blood and volumetric absorptive microsampling

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In this methodology article from Bioanalysis, authors outline the validation of methods for determining pediatric midazolam using wet whole blood and volumetric absorptive microsampling. The authors successful method validation indicates the suitability of volumetric absorptive microsampling as a technique for pediatric clinical studies.

Keywords: hematocrit, volumetric absorptive microsampling, method validation, microsampling, pharmacokinetics.


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Aim:
The collection and quantitative analysis of dry blood using volumetric absorptive microsampling potentially offers significant advantages over conventional wet whole blood analysis. The manuscript published in Bioanalysis explores their use for pediatric sampling and explores additional considerations for the validation of the bioanalytical method.

Results:
HPLC–MS/MS methods for the determination of midazolam and its major metabolite 1-OH midazolam in both whole wet blood, and dry blood collected on VAMS were developed, validated, and used to support an observational clinical study to compare pharmacokinetic parameters in pediatric patients.

Conclusion:

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