Technical Note – Benefits of Differential Ion Mobility Spectrometry for High-Sensitivity Quantification of Peptides

Using the SelexION® Ion Mobility device improves selectivity and sensitivity during biotherapeutic peptide analysis

Evgueni Fedorov
Biotrial Bioanalytical Services Inc., Laval, QC, Canada

Key Challenges of Peptide Quantification

  • Poor MS/MS fragmentation – Cyclic peptides or peptides with disulphide bond have either poor fragmentation or too much fragmentation
  • Lack of sensitivity -Regular MRM which relies on daughter ion fragment has challenges in achieving highly sensitive assay specially in complex biological matrices
  • Selectivity challenges – Parent ion to parent ion quantification, otherwise called Multiple Ion Monitoring (MIM) is the best choice but will encounter very high noise background and matrix interference

Key Features of New Jet Injector SelexION®+ Technology

  • Novel Cell Design – Addition of lens increases ion velocities into cell, reducing transit times through detrimental fringing fields
  • Increased Ion Transmission Efficiency – Potential interferences originating from isobaric compounds present in complex plasma matrix
  • Shorter Ion Residence Time – High background noise in MRM mode  in processed plasma sample
  • Robustness –  Proven to meet regulatory guidelines for inter day and intraday precision and accuracy statistic