Flow cytometric analysis of B, NK and T cell subpopulations in whole blood samples from non-human primate

Written by Iohann Boulay, Selly Hau Yee Hung, Vanessa Plouffe, Karine Blouin, Daniela Andreeva, Julie Mercier, Frédéric Bouchard, Karine Dumaresq-Doiron


While comprehensive assessment of the immune system health often involves the evaluation of major lymphocyte cell lineages (B, NK and T cells), the advent of modern flow cytometry now also allows more
in-depth analysis of these cell subsets. In support of toxicology studies, two staining panels were developed and qualified to monitor naïve (TN), effector memory (TEM) and central memory (TCM) T cytotoxic
and helper cells, as well as B and NK cells, in Non-Human Primate peripheral whole blood.

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