In the Zone: bioanalytical methods for quantifying oligonucleotides
LC–MS enables sensitive and specific measurement of intact oligonucleotides, metabolites and degradation products, supporting pharmacokinetic, biodistribution and toxicology studies. This analytical approach is essential for evaluating therapeutic efficacy and safety in drug development, offering robust data for regulatory submissions and clinical decision-making.
However, the unique structures, modifications and behaviors of oligonucleotide therapeutics present significant analytical challenges that demand specialized approaches and careful method optimization.
This feature brings together expert insights, practical solutions and detailed case studies to help navigate those challenges and achieve reliable oligonucleotide bioanalysis.
[Video by Peter Halliday]
INFOGRAPHIC
LC–MS Analysis of oligonucleotides: a comprehensive guide
LC–MS is essential for evaluating therapeutic efficacy and safety in drug development, offering robust data for regulatory submissions and clinical decision-making, but it is not without its challenges. This infographic breaks down the essential knowledge needed to overcome the unique analytical hurdles that oligonucleotides present. From tackling chromatographic separation challenges to optimizing ionization efficiency, explore expert tips and tricks to help ensure reliable, reproducible LC–MS analysis of oligonucleotides in complex biological matrices.
INTERVIEW
Navigating the bioanalytical complexities of oligonucleotide therapeutics
As oligonucleotides advance from laboratory concepts to clinical realities, the bioanalytical challenges they present have become increasingly complex and critical to therapeutic success. In this interview, Eshani Galermo explores the current landscape of oligonucleotide bioanalysis and provides valuable perspectives on the technological advances and emerging approaches that are shaping the future of oligonucleotide quantitation and characterization in pharmaceutical development.
WEBINAR
A masterclass in advanced analytical assays for bioanalysis of oligonucleotides
There are many challenges when developing bioanalytical methods for the quantitation of oligonucleotides, including stability, recovery, non-specific binding, chromatographic retention and ionization. This webinar presents case studies with various oligonucleotide structures, along with strategies on how to overcome bioanalytical challenges.
RESOURCES
Bioanalytical solutions for drug metabolism and pharmacokinetics
Pathways to a new frontier in bioanalysis
LinkedIn group BioA Collective
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