Agilent: Captiva Filtration

Website: www.agilent.com

Captiva’s unique dual-depth filtration media provides complete removal of precipitated proteins and outstanding resistance to sample clogging, with no loss of analytes. All Captiva components are ultra clean, and rigorously tested to ensure against non-specific binding. With Captiva, your plasma samples are processed quickly and reliably. Captiva is easily automated for enhanced productivity and excellent for sample storage.

Time-consuming sample transfer steps required with conventional precipitation are now a thing of the past. With Captiva, clean, clear filtrates are ready for injection in minutes–this user-friendly filtration device is simple and streamlined with an easy-to-follow 3-step process. And because Captiva samples are pellet-free, you can sample directly from the collection plate.

The Captiva range includes:

  • Captiva NDLipids, the non-drip filtration plate for lipid and protein depletion
  • NEW – Captiva ND is a Non-Drip, fully automated filter plate for protein precipitation
  • Captiva 96-well filter plates for preparing precipitated proteins for LC/MS
  • Captiva filter cartridges, all the usual Captiva benefits in a standard SPE cartridge format

Captiva NDLipids

Improve Analysis by Depleting Phospholipids During Precipitation

Capitiva NDLipids is as simple and easy-to-use as a standard protein precipitation plate. The non-drip 96-well filtration plate is specially designed to effectively remove phospholipids from biofluids. Using Captiva NDLipids removes lipids, proteins, surfactants and other matrix interferences from plasma extracts. Ion suppressions significantly reduced for enhanced sensitivity and precision during trace analysis. The depletion of lipid compounds also gives you better peak shapes and reproducible retention times. In addition, the fast, in-well precipitation technology of Captiva NDLipids ensures high sample throughputs and helps reduce instrumentation downtime in the laboratory, with virtually no need for method development on a wide range of analytes.

Features

  • More precise and reproducible quantitation with removal of phospholipids and proteins
  • Increased productivity due to extended column lifetimes and cleaner MS ion sources
  • Reduced costs and increased productivity due to the decreases in instrument downtime

Captiva ND

Simplify your workflow with Agilent Captiva ND filter plates and get particulate-free protein precipitated samples 5 times faster than centrifugation

The ever increasing sensitivity of today’s MS instruments allows labs to take advantage of the increased speed and productivity of “just enough” techniques such as protein precipitation. Now Agilent introduces Captiva ND non-drip filter plates that are designed to simplify your workflow and make protein precipitation more user-friendly than ever.

Captiva ND’s unique non-drip design simplifies your workflow, ends the need to use messy tips or well seals, and reduces the number of liquid transfer steps needed to process samples. Best of all, Captiva ND’s dual-depth filter construction delivers a fast reproducible flow, so you get uniform sample treatment and reliable filtrate recovery.

Features:

Agilent Captiva ND non-drip filter plates provide:

  • Easy automation: non-drip design resists organic solvent flow until vacuum is applied
  • Exceptional flow: dual depth filter avoids plugged membranes and lost samples
  • Efficient protein removal: MS suitable samples in as little as 1/5th the time

Extends Column Lifetime/Reduces instrument downtime

Negligible effects are seen on system backpressure after prolonged use

Captiva 96-well Filter Kits

Outstanding Resistance to Clogging

Faster than centrifugation and easily automated, Captiva’s unique dual depth filtration media provide complete removal of precipitated proteins and outstanding resistance to sample clogging. With Captiva you can avoid fibrinogen clogging forever. The plates are also excellent for sample storage. All Captiva components are ultra clean, and rigorously tested to ensure against non-specific binding. With Captiva, your plasma samples are processed quickly and reliable. Starter kits contain everything you need to get up and running with minimum fuss. Replacement kits include everything you need to replenish your Captiva system.

Features

  • Rigorous quality control eliminates non-specific binding
  • Fast and reliable processing improves productivity
  • Starter kits contain everything you need

Captiva 96-well Filter Plates

Avoid Clogging Expensive HPLC Columns

Filtration is simple, versatile, and necessary to prevent clogging of expensive HPLC columns. The Captiva 0.2 µm and 0.45 µm depth filter plates are ideal for preparing precipitated protein samples for LC/MS analysis. The Captiva 10 µm and 20 µm glass fiber filter plates are designed for clarifying highly particle-laden samples, such as freshly thawed plasma and hepatocyte filtration, preventing sample transfer problems from pipette tip clogging. They are perfect for automated systems and for use with Duo Seal 96-well seals.

Features

  • Protect HPLC columns from clogging to reduce instrument downtime
  • Clean and clear filtrates offer improved sensitivity
  • High analyte recovery with simple robust methods allows faster method development

Captiva 96-well Collection Plates and Cover

Prevent Sample Contamination

Captiva 96-well Collection Plates are specially designed for use with Captiva Filtration Plates, SPEC SPE 96-well Plates and Bond Elut 96-well Plates. The 1 mL capacity provides the volume needed to collect all of your filtrate or eluate. Captiva Pierceable 96-well Silicone Covers are easily applied to completely seal the plates. This ensures no sample loss due to spillage or evaporation, and no sample contamination. The silicone is specially designed for 96-well auto injectors, providing easy piercing and removal.

Features

  • Designed for Captiva filtration and SPEC, Bond Elut 96 applications
  • Regular 1 mL format offers compatibility with further automation or liquid handling
  • Silicone cover preserves sample integrity

Captiva Filter Cartridges

All the Benefits of Captiva in a Standard SPE Cartridge

Captiva filter cartridges bring all of the benefits of Captiva filtration to the standard SPE cartridge format. The 0.2 µm and 0.45 µm depth filter cartridges are ideal for preparing precipitated protein samples for LC/MS analysis. The Captiva 10 µm glass fiber filter cartridge is designed for clarifying highly particle-laden samples, such as freshly thawed plasma, preventing sample transfer problems due to pipette tip clogging.

Features

  • Standard SPE format
  • Ideal for LC/MS samples
  • Avoid sample transfer problems

 

CaptiVac Vacuum Collars

Prevent Cross Contamination

For use with Captiva Filtration and 96-well Collection Plates and SPEC 96-well Plates, this patented vacuum collar is a completely transparent device that joins Captiva or SPEC plates directly onto our collection plate. The unique design of the Captiva collar forms a pre-set, pre-aligned vacuum seal between the filtration plate and the collection plate, which positions the outlet tips at a specified distance inside each well, so as to prevent cross contamination of samples.

Features

  • Pre-aligned for trouble free operation
  • Vacuum sealed for maximum efficiency
  • Simple, cost effective solution

Agilent offer you solutions to help increase throughput and improve the quality of data. Our Captiva NDLipids non-drip 96-well filtration plate is designed to effectively remove phospholipids from biofluids, significantly reducing ion suppression.

To find out more, please click here.