Technical Note – Quantification of Peptides with Poor MS/MS Fragmentation using Novel Jet Injector SelexION ® + MIM Workflow

Enhanced signal intensities with novel differential mobility cell design

Key Challenges of Peptide Quantification

  • Poor MS/MS fragmentation – Cyclic peptides or peptides with disulphide bond have either poor fragmentation or too much fragmentation
  • Lack of sensitivity -Regular MRM which relies on daughter ion fragment has challenges in achieving highly sensitive assay specially in complex biological matrices
  • Selectivity challenges – Parent ion to parent ion quantification, otherwise called Multiple Ion Monitoring (MIM) is the best choice but will encounter very high noise background and matrix interference

Key Benefits of SelexION®+ Multiple Ion Monitoring workflows

  • Addressing isobaric interferences from complex matrix – SelexION+ reduces matrix interference and background noise originating from isobaric compounds present in complex plasma matrix.
  • Enhanced Selectivity & Sensitivity- With new cell design, SelexION+ provides enhanced sensitivity and specificity without any compromise.
  • Improving Signal-to-Noise Ratio– Reduces high background noise in MRM or MIM in complex matrices such as plasma or tissues.
  • Ensuring better Data quality- SelexION+ improves precision and accuracy statistics at lowest level of quantitation.