Supported liquid extraction versus liquid–liquid extraction for sample preparation in LC–MS/MS-based bioanalysis

"Due to its flow-through property and the large surface area of the interface between the aqueous and organic layer, SLE provides continuous and more efficient extraction than LLE does, resulting in potentially higher recovery." LC-MS/MS is currently the method of choice for bioanalysis of small molecules due to its high selectivity, sensitivity and throughput. However, LC-MS/MS assays often encounter a few common bioanalytical issues such as matrix effect, interference peaks, low recovery and assay reproducibility, resulted from inadequate or inappropriate sample clean-up methods. Among these issues, matrix effect is one of the most complicated and challenging issues to deal with....