Technology Digest: flow cytometry for high-throughput antibody screening
Antibody-based therapeutics, such as monoclonal antibodies (mAbs), have become important treatment modalities for human diseases. Antibody-based drugs have been investigated in a wide range of formats, including polyclonal and hybrid antibodies, but fully human and humanized mAbs are considered the gold standard due to their reduced immunogenicity and high specificity. In recent years, therapeutic antibodies have become best-sellers in the pharmaceutical industry, in part due to a low incidence of adverse events. In order to ensure safety and efficacy of any therapeutic agent, regulators expect products that have been fully characterized throughout the drug discovery process, from hit to lead.
Speed, sensitivity and multiparameter analysis are all considerations when deciding on a screening technique. The sheer volume of potential candidates means that speed is of the essence to screen as many candidates as possible for their binding affinity to the target, but they may be present in a sample at low concentrations. Flexible and sensitive instrumentation is therefore needed to manage the required assay speed and ensure that promising mAbs are identified.
This eBook includes:
- TECHNOLOGY DIGEST: Flow cytometry for high-throughput antibody screening
- BIOANALYTICAL CHALLENGE: Whole blood stability evaluation of monoclonal antibody therapeutics using volumetric absorptive microsampling
- REVIEW: Review of approaches and examples for monitoring biotransformation in protein and peptide therapeutics by MS
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