A new extraction approach for oligonucleotide bioanalysis to enhance recovery, reproducibility and LC-MS sensitivity
Oligonucleotide therapeutics represent a diverse and growing class of drugs with the ability to address disease conditions at the level of gene transcription and translation. As their pipeline expands, the need for quantitative bioanalytical testing via LC–MS has also increased and the challenges to obtaining robust, reproducible quantitative results have become a bottleneck. To address this challenge, Waters is introducing new SPE sample preparation kits that demonstrate improved recovery and reproducibility using a standardized detergent-free protocol that requires little to no method development, is automation friendly and that works across a diverse range of oligonucleotide therapeutics. In this webinar the SPE kits will be introduced and quantitative performance data across a diverse range of oligonucleotide modalities will be presented.
What will you learn?
- Gain an understanding of the LC–MS bioanalytical challenges for oligonucleotides
- How to address critical challenges in establishing an oligonucleotide quantitation workflow: from obtaining selectivity in biological matrices and minimizing oligonucleotide loss due to solubility and non-specific adsorption, to achieving high oligonucleotide extraction efficiency, LC-MS sensitivity and robustness
- How implementation of a new SPE kit-based approach, with QC verified, lot-traceable reagents and standardized protocol, reduces sample preparation complexity, minimizes method development, improves repeatability and facilitates high throughput sample processing
Who may this interest?
- Pharma/Biotech companies developing oligotherapeutics who are engaged in preclinical and/or clinical studies that require extraction and trace level quantification of these drug candidates from biological matrices
- CROs who offer oligonucleotide bioanalytical services in support of pharma drug development
- Academic principal investigators and scientists engaged in oligotherapeutic research and development
- Method development sample preparation scientists, lab managers and lab directors who run bioanalytical labs
Speakers
Joe Fredette
Senior Product Marketing Manager – Nucleic Acid Therapeutics
Waters Corporation (MA, USA)
Joe Fredette is a Senior Product Marketing Manager at Waters where he is focused on the development and commercialization of analytical tools and solutions for nucleic acid therapeutics (cell and gene therapy/ new modalities). Joe received a BS in Microbiology from Cornell University (NY, USA) and has worked in the life sciences industry throughout his career. He joined Waters in 2014 bringing with him broad hands-on experience leading the development and commercialization of life science tools and solutions.
Mary Trudeau
Consulting Scientist and Manager of Sample Preparation Group
Waters Corporation (MA, USA)
Mary Trudeau is a Consulting Scientist and Manager for the Sample Preparation Group within the Consumable and Lab Automation business segment. Following a successful career in pharma with Pfizer (NY, USA), Mary joined Waters in 2011. She is responsible for developing and optimizing methods and workflows to address customer pain points across multiple market segments. Her team’s primary focus has been developing and highlighting automated sample preparation of bioanalytical (ADME/DMPK) workflows for both small and large molecules.
This webinar was recorded on the 19th of October 2023.
In association with
