Reaching the next level of sensitivity in oligonucleotide bioanalysis: a hybridization-based method on Quanterix Simoa®
In this webinar, we will present a case study where a hybridization assay was developed on the Simoa® for oligonucleotides. Challenges faced during method establishment on this ultra-sensitive immunoassay technology will be discussed such as labeling of paramagnetic beads, assay reproducibility and precision, stability of conjugated paramagnetic beads, along with approaches taken to overcome and/or mitigate.
To evaluate the gains in sensitivity, the Simoa® assay will be compared to a more conventional hybridization assay approach using electrochemiluminescence (ECL) for detection of the same oligonucleotide therapeutic and the limits of quantitation achieved.
What will you learn?
- To understand the mechanism of Simoa® technology for detection of oligonucleotide therapeutics in biological matrices
- Realize the potential gains in sensitivity that can be achieved by running a hybridization assay on the Simoa® platform in comparison to a conventional approach using ECL
- Appreciate the potential applications of Simoa® for evaluation of pharmacokinetics of biologic and nucleic acid therapeutics, as well as some of the challenges that will be faced
Who may this interest?
- Pharma and Agile Biotech clients who work with oligonucleotide therapeutics
- Bioanalytical scientists performing PK/PD assessments of oligonucleotides
- Instrument companies
Speaker
Jill Uhlenkamp, PhD
Senior Method Development Manager, Immunochemistry
Labcorp Drug Development (IN, USA)
Dr Jill Uhlenkamp has nearly 20 years of experience in immunoassay development, with more than a decade spent in regulated bioanalysis for drug development, including in both biotech and CRO settings.
She has developed or overseen development and validation for a wide range of bioanalytical assays. Her experience includes PK, ADA, NAb and biomarker assays, preclinical and clinical studies and small, large and oligonucleotide-based therapeutics in a range of matrices.
At Labcorp Bioanalytical Services, Jill currently serves as the senior method development manager, overseeing the immunochemistry method development and method validaton teams at the Indianapolis site. Jill earned her PhD in analytical chemistry from Iowa State University (IA, USA).
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