Publication / Source: Bioanalysis 3(12)
Authors: Ahn DR
“When multiplex immunoassays are rigorously assessed regarding accuracy and efficiency for the clinical use, there is no doubt that these methods will be utilized in the diagnosis, management and risk prediction of various diseases.”
Immunoassays utilizing the specific interaction between antigens and antibodies have been widely used for quantitative detection of various analytes in life sciences. ELISA is a microwell plate-based assay and has been the most commonly used immunoassay format for a long time. ELISA still remains the gold standard for quantifying protein markers in human body fluids, particularly blood. Abnormal levels of the markers measured by means of ELISA have the potential to be used as diagnostic indicators. Diagnosis of a disease with a single marker, however, seems nearly impossible because of the complexity of disease-causing pathways in the biological system.