Bioanalysis Zone

A toxicological perspective on the use of spin column extraction to detect drugs and metabolites in biological matrices

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 “…commercially available columns or devices for monoliths are still limited compared with SPME. In the future, monoliths are expected to be used for the extraction and purification of a wider range of analytes in various fields.”

A very important objective in investigations on toxicological and pharmaceutical properties of drugs, medicines and metabolites is their extraction from biological matrices. The last three decades have witnessed several advancements in analytical instrumentation. Liquid–liquid extraction or SPE methods are widely used for the extraction, purification and enrichment of drugs and medicines from biological materials; however, these methods involve laborious, intensive and expensive preparatory procedures. Furthermore, organic solvents used in these methods are toxic to both humans and the environment. Therefore, recent investigations have focused on methods to reduce the sample volume and analytical time, and eliminate the use of chlorinated solvents. Solid-phase microextraction (SPME) techniques are novel and advantageous: they require a small sample volume, are easy to perform, enable rapid detection, do not require a solvent [1] and can be applied for biological analyses [2].

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