Case study: automating ELISA-based assays to assess the purity of therapeutic viral vectors


In this case study, the analytical development group at AGC Biologics (Milan, Italy) describes the automation of two ELISA-based workflows to assess lentiviral vector purity, using a Hamilton Microlab® STARlet liquid handler integrated with a plate washer, a plate spectrometer and two plate incubators.  

Highlights of this case study: 

  • Overview of the analytical methods automated by AGC Biologics Milan on Hamilton liquid handlers
  • Detailed description of two ELISA-based automated workflows to assess the purity of the lentiviral vectors: residual benzonase and residual host-cell proteins
  • Advantages of the Hamilton VENUS Scheduler to plan and execute parallel assays using the same sample aliquot

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