Application Note: Compound identification, profiling and pathway analysis of the yeast metabolome in Mass Profiler Professional (Agilent)

A nontargeted, discovery-based approach for analyzing the yeast metabolome in response to calcium and immunosuppressant drug treatment was demonstrated using multiple LC conditions and the Agilent 6530 Accurate Mass Q-TOF mass spectrometer. Data analysis using Mass Profiler Professional (MPP) and the Personal Compound Database & Library (PCDL) yielded putative identifications for several hundred compounds, many of which were observed to be differential between controls and treated groups. Furthermore, canonical biological pathways analysis was performed using Pathway Architect to efficiently project the results of differential abundance results onto publicly available biological pathways, including BioCyc, MetaCyc, and WikiPathways. We inferred the importance of several metabolites using a pathway analysis strategy where we interactively visualized and analyzed metabolites that were over-represented in known biochemical pathways.

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