Blood cell quantification on dry blood samples: toward patient-centric complete blood counts


Dobielewski M, Hauser J, Beck O, Stemme G & Roxhed N | Bioanalysis, 14(10), 693-701, (2022)

Keywords: • blood • cells • complete blood count • counting • differentiation • dry • patient-centric • quantification • RBC • WBC

Background: Performing complete blood counts from patients’ homes could have a transformative impact on e-based healthcare. Blood microsampling and sample drying are enabling elements for patient-centric healthcare. The aim of this study was to investigate the potential of dry blood samples for image-based cell quantification of red and white blood cells. Methods: A manual sample preparation method was developed and tested for image-based red and white blood cell counting. Results & conclusion: Dry blood samples enable image-based cell counting of red and white blood cells with a good correlation to gold standard hematology analyzer data (average coefficient of variation <6.5%; R2 >0.8) and resolve the basic morphology of white blood cell nuclei. The presented proof-of-principle study is a first step toward patient-centric complete blood counts.

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