Combining LC–MS/MS and hollow-fiber infection model for real-time quantitation of ampicillin to antimicrobial resistance

Urinary tract infections are becoming untreatable with the rise of antibiotic resistance. Escherichia coli is the cause of most urinary tract infections, one of the most common infections that require treatment with antibiotics. The utility of a hollow-fiber system, which is a dynamic system mimicking human disposition of antibiotics more closely than static systems, can help design single or combination drug dosing regimens for antibiotics based on their pharmacokinetic profiles. The data generated from these studies can be utilized to help understand the time when bacteria start developing resistance and aid in adjusting the drug combinations and their doses. The...