Masterclass: comprehensive LC–MS workflows for ADC analysis

This presentation presents a streamlined and sensitive LC–MS workflow for four key quantitative pharmacokinetic/pharmacodynamic (PK/PD) assays using trastuzumab deruxtecan (TDx) in rat plasma on a triple quad mass spectrometer. Each of these assays provides unique insights into the behavior of the antibody drug conjugate (ADC), enabling a comprehensive PK/PD evaluation. Sensitivity and quantitative performance are critical for ADC analysis, enabling accurate and thorough assessments of drug stability, safety and therapeutic efficacy. The session will highlight assay sensitivity, method robustness and applicability to bioanalysis.