Sensitive quantification of mAbs at the signature peptide level in complex matrices using accurate mass spectrometry
In this webinar, we will discuss a case study demonstrating a highly sensitive microflow approach to quantify trastuzumab and pertuzumab in human serum on a high-end, accurate mass spectrometer. This method utilizes a simple immunoprecipitation and on-bead digestion for measurement of signature peptides. Assay performance indicates that the workflow could be applied in pharmacokinetic studies that are limited by low sample volumes and complex matrices.
What will you learn?
- Achieve higher selectivity and sensitivity for mAb in human serum using a quadrupole time-of-flight (QTOF) mass spectrometer
- Acquire highly sensitive results with low sample volumes by leveraging instrument capabilities to maximize MS/MS sampling efficiency
- Employ a microflow workflow for quantification to enhance sampling efficiency and achieve previously unattainable lower limits of quantification (LLOQs) at the signature peptide level
Who may this interest?
- Researchers in drug discovery and development
- Drug developers performing pharmacokinetic studies and regulated bioanalysis
Speaker
Simon Roberts
Staff Application Scientist
SCIEX (MA, USA)
Simon Roberts is an Application Scientist at SCIEX (MA, USA), where he has worked for 8 years. Simon completed his PhD at Duke University (NC, USA) and his postdoctoral fellowship at Colorado School of Mines (CO, USA) where he studied nontarget identification of environmental contaminants such as pesticides and PFAS. Simon currently supports application development and customer support in both nominal mass and high-resolution mass spectrometry. He is an avid trail runner and competes in ultra-marathon running events around the country.
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